Abstract
Barley microspore-derived doubled-haploid embryos have been produced in vitro. The development of embryo desiccation technology will allow long-term storage, germplasm preservation and low delivery cost. Treatment of the microspore-derived embryos was essential to induce desiccation tolerance and to arrest further development and plant regeneration. At the concentrations used, a treatment with trehalose was more efficient than with sucrose, and mannitol was harmful to the embryos. Up to 80% of the desiccated embryos produced complete green plants when transferred to regeneration medium, by the application of a 0.6 m trehalose or a 10–5 m abscisic acid treatment to the embryos in the culture induction medium. The morphology of these plants was similar to plants produced directly from non-desiccated embryos.
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Received: 28 September 1998 / Revision received: 27 November 1998 / Accepted: 5 January 1999
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Ryan, A., Castillo, A., Vallés, M. et al. Desiccated doubled-haploid embryos obtained from microspore culture of barley cv. Igri. Plant Cell Reports 18, 924–928 (1999). https://doi.org/10.1007/s002990050685
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DOI: https://doi.org/10.1007/s002990050685