Abstract
Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400–800 mg/l) when 5–10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cells into the liquid medium, and an excess of berberine crystallized as its nitrate in the medium. When the cells were cultured in a modified LS medium containing 20 mM KNO3 and 40 mM NH4Cl in place of 20.6 mM NH4NO3 as nitrogen source, most of the alkaloid crystallized to form berberine chloride instead of nitrate. Minor alkaloids, thalifendine and magnoflorine, were also isolated from the medium and identified.
Similar content being viewed by others
Abbreviations
- LS:
-
Linsmaier and Skoog (1965)
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
1-naphthaleneacetic acid
- BA:
-
6-benzyladenine
References
Fukui H, Nakagawa K, Tsuda S, Tabata M In: Fujiwara A (ed) Plant Tissue & Cell Culture 1982. Proc. 5th Intl. Cong. Plant Tissue & Cell Culture pp. 313–314
Hinz H, Zenk MH (1981) Naturwiss. 67: 620–621
Ikuta A, Itokawa H (1982) Phytochemistry 21: 1419–1421
Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127
Misaki T, Sagara K, Ojima M, Kakizawa S, Oshima T, Yoshizawa H (1982) Chem. Pharm. Bull. 30: 354–357
Sato F, Yamada Y (1984) Phytochemistry, 23: 281–285
Tsukada M, Tabata M (1984) Planta Medica, 51: 338–341
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Nakagawa, K., Konagai, A., Fukui, H. et al. Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Reports 3, 254–257 (1984). https://doi.org/10.1007/BF00269306
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00269306