Abstract
The selection and characterization of 5-methyltryptophan (5-MT) resistant cell lines is described in a dihaploid potato, clone H2578(007). The frequency of resistant calli was increased by treatment of a cell suspension with N-ethyl-N-nitroso-urea. Two lines, 5-MT-21 and 5-MT-26, accumulated tyrosine (160 nMol and 1546 nMol/g fresh callus), and the third, 5-MT-27, accumulated both tyrosine (841 nMol/g fresh callus) and phenylalanine (451 nMol/g fresh callus). In the wildtype tyrosine and phenylalanine content was 65 nMol and 42 nMol/g callus, respectively. The tryptophan content of line 5-MT-26 was significantly increased, from 20 nMol/g to 76 nMol/g fresh callus. The total free amino acid content of the three variant cell lines was higher than that of the wildtype. Variant cell lines 5-MT-21, -26 and -27 showed a low degree of resistance to 5-MT, when grown on a selective medium and were cross-resistant to parafluorophenylalanine and 3-fluorotyrosine, analogues of phenylalanine and tyrosine.
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Abbreviations
- ENU:
-
N-ethyl-N-nitroso-urea
- 5-MT:
-
5-methyltryptophan
- 3-FT:
-
3-fluorotyrosine
- PFP:
-
parafluorophenylalanine
- AEC:
-
S-(2-amino-ethyl) L-cysteine
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Communicated by P. Maliga
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Jacobsen, E., Visser, R.G.F. & Wijbrandi, J. Phenylalanine and tyrosine accumulating cell lines of a dihaploid potato selected by resistance to 5-methyltryptophan. Plant Cell Reports 4, 151–154 (1985). https://doi.org/10.1007/BF00571304
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DOI: https://doi.org/10.1007/BF00571304