Detection of Cryptosporidium parvum and Cryptosporidium muris in soil samples

Abstract

 The flotation procedure for the detection of Cryptosporidium parvum and Cryptosporidium muris oocysts in feces was adapted for use on soil samples. Soil samples were seeded with known amounts of purified C. parvum or C. muris oocysts and Cryptosporidium spp.-free bovine feces. The limit of detection for this procedure was determined at different levels of inoculation for each species. At each level of inoculation, 30 control samples were processed and the observer was blind to the status of the sample. All samples were examined for the presence of Cryptosporidium spp. oocysts using phase-contrast microscopy. The samples were seeded with the following estimated counts of C. parvum oocysts: 1000/g, 1250/g, and 1500/g. These levels had sensitivities of 88%, 90%, and 93%, respectively. All inoculation levels had a specificity of 100%. Thirty additional samples were inoculated with C. muris and the limit of detection was found to be 76 oocysts/g sample, with a sensitivity of 97% and a specificity of 100%.