Summary
The fine structure of right sciatic nerves of Swiss white mice was studied after being exposed to a venom solution ofAgkistrodon contortrix laticinctus (broad-banded copperhead) and to 0.9% NaCl solutions for periods of 1, 2 or 3 h. There was some “Wallerian-like” degeneration in all groups of nerves in the experiment. Additional neuropathological changes were noted in the nerves exposed to venom solutions.
The connective tissue was affected most severely, while the basal lamina was unaffected. Schwann cells were vacuolated, and in the most extreme case, a type of myelin splitting occurred which was considered as a “demyelinative” effect. The results were compared to those of Martin and Rosenberg (1968) [15] on studies of the giant squid axon. References to additional fine structural studies of various other neuropathies were given.
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References
Appenzeller, O., Kornfeld, M., MacGee, J.: Neuropathy in chronic renal disease. A microscopic, ultrastructural and biochemical study of sural nerve biopsies. Arch. Neurol. (Chic.)24, 449–461 (1971).
Blümcke, S., Themann, H., Niedorf, H. R.: Deposition of glycogen in degeneration and regeneration of the sciatic nerves of rabbits. Acta neuropath. (Berl.)5, 69–81 (1965).
Cheymol, J., Bourillet, F., Roch-Arveiller, M.: Action neuromusculaire des venins de quelques crotalidae, elapidae et hydrophiidae. Mem. Inst. Butantan33, 541–554 (1966).
———: Actions neuromuscularies comparees des venins de trois naja. Arch. int. Pharmacol.170, 193–215 (1967)
Condrea, E., Rosenberg, P.: Demonstration of phospholipid splitting as the factor responsible for increased permeability and block of axonal conduction induced by snake venom. II. Study on squid axons. Biochim. biophys. Acta (Amst.)150, 271–284 (1968).
—— Dettbarn, W. D.: Demonstration of phospholipid splitting as the factor responsible for increased permeability and block of axonal conduction induced by snake venom. I. Study on lobster axons. Biochim. biophys. Acta (Amst.)135, 669–681 (1967).
Davison, A. M., Peters, A.: Myelination. Kugelmass, I. Newton, eds., pp. 7–36. Springfield, Ill.: Ch. C. Thomas 1970.
Duncan, C., Strub, R., McGarry, P., Duncan, D.: Peripheral nerve as an aid to diagnosis in infantile neuroaxonal dystrophy. Neurology (Minneap.)20, 1024–1032 (1970).
Fahmy, A.: An extemporaneous lead citrate stain for electron microscopy. Proc. Electron Microsc., Soc. Amer., pp. 148–149. Baton Rouge, La.: Claitor's Book Store 1969.
Jacobson, S.: Electrophysical and ultrastructural changes in isolated sciatic nerves. Exp. Neurol.13, 22–39 (1965).
Lampert, P. W., Schochet, S. S.: Demyelination and remyelination in lead neuropathy. J. Neuropath. exp. Neurol.27, 527–545 (1968).
Lee, J. C.-Y.: Electron microscopy of Wallerian degeneration. J. comp. Neurol.120, 65–71 (1963).
Litchfield, J. T., Jr., Wilcoxon, F.: A simplified method of evaluating dose effect experimentally. J. Pharmacol. exp. Ther.96, 99–113 (1949).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414 (1961).
Martin, R., Rosenberg, P.: Fine structural alterations associated with venom action on squid nerve fibers. J. Cell Biol.36, 341–353 (1968).
Masurovsky, E. B., Bunge, M. B., Bunge, R. P.: Cytological studies of organotypic cultures of rat dorsal root ganglia following X-irradiationin vitro. J. Cell Biol.32, 497–518 (1967).
Morazain, R., Rosenberg, P.: Lipid changes in triothocreasyl phosphate-induced neuropathy. Toxicol. appl. Pharmacol.16, 461–474 (1970).
Raine, C. S., Bornstein, M. B.: Experimental allergic encephalomyelitis. An ultrastructural study of experimental demyelinationin vitro. J. Neuropath. exp. Neurol.29, 177–191 (1970).
Rosenberg, P.: The use of snake venoms as pharmacological tools in studying nerve activity. Neuropoisons: Their pathophysiological actions. L. Simpson, ed., pp. 111–136. New York: Plenum Press 1970.
— Ng, K. Y.: Factors in venom leading to block of axonal conduction by curare. Biochim. biophys. Acta (Amst.)75, 116–128 (1963).
Stringer, J. M., Kainer, R. A., Tu, A. T.: Ultrastructural studies of myonecrosis induced by cobra venom in mice. Toxicol. appl. Pharmacol.18, 442–450 (1971).
Stringer, J. M., Kainer, R. A., Tu, A. T.: Myonecrosis induced by rattlesnake venom: An electron microscope study. Amer. J. Path. (in press) (1972).
Swank, R. L.: A biochemical basis of multiple sclerosis. Springfield, Ill.: Ch. C. Thomas 1961.
Wiśniewski, H., Prineas, J., Raine, C. S.: An ultrastructural study of experimental demyelination and remyelination. I. Acute experimental allergic encephalomyelitis in the peripheral nervous system. Lab. Invest.21, 105–118 (1969).
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This investigation was submitted as a partial requirement for the Master of Science Degree, Department of Anatomy, Colorado State University and was supported by U.S. Public Health Service Grant # 5 RO1 GM-15591 and # 5 RO1 FD-00014 and Career Development Award # 5 KO4 GM-41786 from the National Institutes of Health (to A. T. Tu). The work was done in the Electron Microscope Training Laboratory, Colorade State University.
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Schmidt, M.E., Abdelbaki, Y.Z. & Tu, A.T. Fine structural changes of myelinated nerve associated with copperhead envenomation. Acta Neuropathol 21, 68–75 (1972). https://doi.org/10.1007/BF00688001
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DOI: https://doi.org/10.1007/BF00688001