Summary
After sympathetic denervation of the guinea-pig distal colon the time course and the relationship between morphological and functional changes were studied, by using the fluorescence method and by evaluating the response of longitudinal muscle to periarterial nerve stimulation.
The inhibitory response to sympathetic nerve stimulation was completely abolished 48 h after denervation. Tissue catecholamines can no longer be detected in either the intramural vessels or in the intramural plexuses 96 h after denervation, and in no preparation could they be observed again until 24 days after denervation.
After 30 days catecholamines stores slowly began to reappear and were completely restored only 100–120 days after denervation. During the whole period of reinnervation the response to periarterial nerve stimulation was lacking.
The inhibitory effect could be elicited only 130 days after denervation and before the morphological pattern had been completely restored.
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Some of the results were communicated at the 33rd Congress of the German Pharmacological Society—Heidelberg 27–30 September 1970 and appeared earlier in Arch. Pharmak.269, n. 2-4, 387 (1971).
Suported by a grant of the Consiglio Nazionale delle Richerche (Roma).
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Mazzanti, L., del Tacca, M., Breschi, M.C. et al. The time course of functional and morphological changes of the guinea-pig colon after “a frigore” denervation of the periarterial sympathetic nerves. Acta Neuropathol 22, 190–199 (1972). https://doi.org/10.1007/BF00684522
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DOI: https://doi.org/10.1007/BF00684522