Summary
Explants of a human sacral chordoma were successfully maintained on collagen-coated coverslips, gelfoam sponge matrices, and Millipore filter platforms for up to 30 days. Tumor cells cultured on collagen-coated coverslips became increasingly vacuolated whereas those maintained in organ culture were entirely free of vacuoles after 22 days in vitro. A single basic tumor cell, small and polygonal with a large central spherical nucleus and abundant endoplasmic reticulum and Golgi apparatus, was recognized. Vacuoles were formed as the result of the progressive expansion of smooth endoplasmic reticulum. Coalescence of these vacuoles produced the physaliferous cell of light microscopy.
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Botticelli, A., Pavetto, C., Pozzi, F., Sannicola Botticelli, C.: Sul cordoma sacrococcigeo. Aspetti istomorfologici e istochimici. Arch. De Vecchi Anat. pat.53, 577–596 (1968)
Cancilla, P., Morecki, R., Hurwitt, E. S.: Fine structure of a recurrent chordoma. Arch. Neurol. (Chic.)11, 289–295 (1964)
Capelli, A., Jasonni, V., Pizzoferrato, A.: Profilo morfologico ed istochimico dei cordomi. Arch. ital. Anat. Ist. pat.42, 46–92 (1968)
Cappell, D. F.: Chordoma of the vertebral column with three new cases. J. Path. Bact.31, 797–814 (1928)
Cesarini, J.-P., Bonneau, H.: A propos de l'étude ultrastructurale d'un chordome sacré. Arch. Anat. path.19, 95–102 (1971)
Congdon, C. C.: Proliferative lesions resembling chordoma following puncture of nucleus pulposus in rabbits. J. nat. Cancer Inst.12, 893–907 (1952)
Crawford, T.: The staining reactions of chordoma. J. clin. Path.11, 110–113 (1958)
Dahlin, D. C., MacCarty, C. S.: Chordoma: A study of 59 cases. Cancer5, 1170–1178 (1952)
Erlandson, R. A., Tandler, B., Lieberman, P. H., Higinbotham, N. L.: Ultrastructure of human chordoma. Cancer Res.28, 2115–2125 (1968)
Friedmann, I., Harrison, D. F. N., Bird, E. S.: The fine structure of chordoma with particular reference to the physaliphorous cell. J. clin. Path.15, 116–125 (1962)
Gessaga, E. C., Mair, W. G. P., Grant, D. N.: Ultrastructure of a sacrococcygeal chordoma. Acta neuropath. (Berl.)25, 27–35 (1973)
Harvey, W. F., Dawson, E. K.: Chordoma. Edinb. med. J.48, 713–730 (1941)
Horwitz, T.: Chordal ectopia and its possible relation to chordoma. Arch. Path.31, 354–362 (1941)
Kay, S., Schatzki, P. F.: Ultrastructural observations of a chordoma arising in the clivus. Human Path.3, 403–413 (1972)
Müller, H.: Über das Vorkommen von Resten der Chorda dorsalis bei Menschen nach der Geburt und über ihr Verhältnis zu den Gallertgeschwülsten am Clivus. Z. rat. Med.2, 202–229 (1858)
Murad, T. M., Murthy, M. S. N.: Ultrastructure of a chordoma. Cancer25, 1204–1215 (1970)
Peña, C. E., Horvat, B. L., Fisher, E. R.: The ultrastructure of chordoma. Amer. J. clin. Path.53, 544–551 (1970)
Pinchuk, V. G., Svigun, V. S., Monastyrskaia, B. D.: Electron microscopic characteristics of caudal chordoma. Arkh. Pat.31, 38–42 (1969)
Rawlinson, D. G., Rubinstein, L. J., Herman, M. M.: In vitro characteristics of a myxopapillary ependymoma of the filum terminale maintained in tissue and organ culture systems. Acta neuropath. (Berl.)27, 185–200 (1974)
Ribbert, H.: Über die Ecchondrosis physalifora sphenooccipitalis. Zbl. allg. Path. path. Anat.5, 457–461 (1894)
Ribbert, H.: Über die experimentelle Erzeugung einer Ecchondrosis physalifora. Verh. Kong. inn. Med.13, 455–464 (1895)
Rubinstein, L. J., Herman, M. M.: Studies on the differentiation of human and experimental gliomas in organ culture system. In: Recent results in cancer research, vol. 51 (ed. J. Hekmatpanah). Berlin-Heidelberg-New York: Springer 1975
Rubinstein, L. J., Herman, M. M., Foley, V. L.: In vitro characteristics of human glioblastomas maintained in organ culture systems. Light microscopy observations. Amer. J. Path.71, 61–76 (1973)
Soffer, D., Brucher, J. M., Wechsler, W.: Zur Feinstruktur menschlicher Chordome. Path. europ.5, 420–441 (1970)
Spjut, H. J., Luse, S. A.: Chordoma: An electron microscopic study. Cancer17, 643–656 (1964)
Tripier, M. F., Hassoun, J., Toga, M.: Étude ultrastructurale d'un chordome. J. neurol. Sci.25, 361–370 (1975)
Virchow, R.: Untersuchungen über die Entwicklung des Schädelgrundes im gesunden und krankhaften Zustande und über den Einfluß derselben auf Schädelform, Gesichtsbildung und Gehirnbau. Berlin: G. Reimer 1857
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Supported by Research Grant CA-11689 from the National Cancer Institute and Graduate Neuropathology Training Grant 5T01 NS 5500-10 from the National Institute of Neurological Diseases and Stroke, U.S.P.H.S.
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Horten, B.C., Montague, S.R. In vitro characteristics of a sacrococcygeal chordoma maintained in tissue and organ culture systems. Acta Neuropathol 35, 13–25 (1976). https://doi.org/10.1007/BF00688940
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DOI: https://doi.org/10.1007/BF00688940