Summary
In 32 cases of human neurological disorders, including 19 cases of Multiple Sclerosis, an analysis was performed on formalin fixed paraffin embedded brain tissue, by using fluorescein labelled antibodies and Nomarski optics. This method, which allows the reduction of technical artifarcts, permitted to establish the presence of intracytoplasmic immunoglobulin binding lymphoid cells with IgG and IgA, more rarely IgM, in the majority of the multiple sclerosis cases. This supports the theory of the intrathecal secretion of antibodies within the central nervous system but does not allow to explain the function of those B. Lymphocytes. The same immunological picture was found in Subacute Sclerosing Panencephalitis. On the contrary, it was different in Progressive Multifocal Leucoencephalopathy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aarli, J. A., Aparicio, S. R., Lumsden, C. E., Tonder, O.: Binding of normal human IgG to myelin sheaths, glia and neurons. Immunology28, 171–185 (1975)
Allerand, C. D., Yahr, M. D.: Gammaglobulin affinity for normal human tissue of the central nervous system. Science144, 1141–1142 (1964)
Castaigne, P., Escourolle, R., Brunet, P., Gray, F., Rouques, C., Le Bigot, P.: Reticulosarcome cérébral primitif associé à des lésions de sclérose en plaques. Rev. Neurol.130, 181–188 (1974)
Castaigne, P., Escourolle, R., Derouesne, C., Cathala, F., Hauw, J. J., Duclos, M., Goust, J. M.: Un cas de leucoencéphalopathie multifocale progressive. Etude anatomo-pathologique, clinique et immunologique. Isolement à partir d'une biopsie cérébrale d'une nouvelle souche de virus Papova. Rev. Neurol.128, 85–95 (1973)
Castaigne, P., Rondot, P., Escourolle, R., Ribadeau Dumas, J. L., Cathala, F., Hauw, J. J.: Leucoencéphalopathie multifocale progressive et gliomes multiples. Rev. Neurol.130, 9–10, 379–392 (1974)
Dubois-Dalq, M., Schumacher, G., Worthington, E. K.: Immunoperoxydase studies on multiple sclerosis brain. Neurology25, 496 (1975)
Esiri, M. M., Taylor, C. R., Mason, D. Y.: Application of an immunoperoxydase method to a study of the central nervous system: preliminary findings in a study of human formalin fixed material. Neuropath. Appl. Neurobiol.2, 233–246 (1976)
Frick, E.: Zur Pathogenese entzündlicher Nervenerkrankungen. Über die Bedeutung immunkompetenter Zellen. Fortschr. Med.87, 1191–1194 (1969)
Guseo, A., Jellinger, K.: The significance of Perivascular Infiltrations in Multiple Sclerosis. J. Neurol.211, 51–60 (1975)
Huang, S., Minassian, H., More, J. D.: Application of immunofluorescent staining on paraffine sections improved by trypsin digestion. Lab. Invest.35, 383–390 (1976)
Kabat, E. A., Moore, D. M., Landow, H.: An electrophoretic study of the protein components in the cerebrospinal fluid and their relationships to serum proteins. J. Clin. Invest.21, 571–585 (1942)
Lebar, R., Voisin, G. A.: Studies on autoimmune encephalomyelitis in the guinea pig. I. Influence of different immunisations on antibody specificity and biological properties, relation to the incidence of the disease. Int. Arch. Allergy appl. Immunol.46, 82–103 (1974)
Link, H.: Oligoclonal immunoglobulin G in multiple sclerosis brain. J. Neurol. Sci.16, 103–114 (1972)
Lumsden, C. E.: Immunogenesis of the multiple sclerosis plaque. Brain Res.28, 365–390 (1971)
Maranetti, G. V., Pettit, D.: The interaction of gammaglobulin with lipids. Chem. Phys. Lipids2, 17–34 (1968)
Murphy, M. J., Hay, J. B., Morris, B., Bessis, M.: Ultrastructural analysis of antibody synthesis in cells from lymph and lymph nodes. Am. J. Path.66, 25–42 (1972)
Mussini, J.-M., Hauw, J. J., Escourolle, R.: Combined Nomarski interference contrast and immunofluorescent study of neuropathological specimens=CSF sediments and paraffin embedded brain tissues. Submitted for publication.
Mussini, J.-M., Hauw, J.-J., Delasnerie, N., Schuller, E., Lhermitte, F., Castaigne, P., Escourolle, R.: Intracytoplasmic immunoglobulin binding lymphoid cells (CILC) of the cerebrospinal fluid in multiple sclerosis and other neurological disorders. Biomedicine (in press)
Papahadjolopoulos, D., Kimelberg, H. K.: Phospholipid vesicles (liposomes) as models for biological membranes: their properties and interactions with cholesterol and proteins. In: Progress in Surface Science, 4, 141–232 (ed. S. G. Davidson). Elmsfort, New York: Pergamon Press
Powers, J. M., McKeever, P. E.: Central pontine myelinolysis: an ultrastructural and elemental study. J. Neurol. Sci.29, 65–81 (1976)
Prineas, J. W., Raine, C. S.: Electron microscopy and immunoperoxydase studies of early multiple sclerosis lesions. Neurology (Minneap.)26, 29–32 (1976)
Sainte-Marie, G.: A paraffine embedding technique for studies employing immunofluorescence. J. Histochem. Cytochem.10, 250–256 (1962)
Sandberg-Wollheim, M., Turesson, I.: Lymphocyte subpopulation in the cerebrospinal fluid and peripheral blood in patients with multiple sclerosis. Scand. J. Immunol.4, 831–836 (1975)
Simpson, J. F., Tourtelotte, W. W., Kokmen, E.: Fluorescent protein tracing in multiple sclerosis brain tissue. Arch. Neurol. (Chicago)20, 373–377 (1969)
Tanaka, R., Iwasaki, Y., Koprowski, M.: Ultrastructural studies of perivascular cuffing cells in multiple sclerosis. Am. J. Path.81, 467–478 (1975)
Tavolato, B. F.: Immunoglobin G distribution in multiple sclerosis brain. An immunofluorescence study. J. Neurol. Sci.24, 1–11 (1975)
Ter Meulen, V., Enders-Ruckle, G., Müller, D., Joppich, G.: Immunhistological, microscopical and neurochemical studies on encephalitides. III: subacute progressive panencephalitis. Virological and immunhistological studies. Acta neuropath. (Berlin)12, 244–259 (1969)
Tourtelotte, W. W.: Interaction de l'immunité locale du système nerveux central et de l'immunité générale au cours de la sclérose en plaques. Rev. Neurol. (Paris)121, 497–504 (1972)
Tourtelotte, W. W., Kokmen, E., Tavolato, B., Kruger, P. S.: Fluorescent cell tracing in multiple sclerosis in brain tissue. Trans. Am. Neurol. Assoc.96, 318–320 (1971)
Vandvik, B., Reske-Nielsen, E.: Immunochemical and immunohistochemical studies of brain tissue in subacute sclerosing panencephalitis and multiple sclerosis. Acta neurol. Scand.48, supp. 51, 413–416 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mussini, J.M., Hauw, J.J. & Escourolle, R. Immunofluorescence studies of intra cytoplasmic immunoglobulin binding lymphoid cells (CILC) in the central nervous system. Report of 32 cases including 19 multiple sclerosis. Acta Neuropathol 40, 227–232 (1977). https://doi.org/10.1007/BF00691958
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00691958