Summary
Acute canine distemper virus (CDV)-induced demyelinating lesions were examined with double-labelling immunocytochemistry simultaneously demonstrating CDV antigen and glial fibrillary acidid protein (GFAP) as marker for astrocytes. It was shown that 64% of all astrocytes within the demyelinating lesions were infected and that 95% of all infected cells counted in the lesions were astrocytes. These results suggest that the astrocyte ist the main target for CDV and that astroglial infection may play an important role in the mechanism of demyelination.
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References
Bloch B, Popovici T, Levin MJ, Tuil D, Kahn A (1985) Transferrin gene expression visualized in oligodendrocytes of the rat brain by using in situ hybridization and immunohistochemistry. Proc Natl Acad Sci USA 82:6706–6710
Bollo E, Zurbriggen A, Vandevelde M, Fankhauser R (1986) Canine distemper virus clearance in chronic inflammatory demyelination. Acta Neuropathol (Berl) 72:69–73
Fischer K (1965) Einschlußkörperchen bei Hunden mit Staupe-Enzephalitis und anderen Erkrankungen des Zentralnervensystems. Pathol Vet 2:380–410
Higgins RJ, Krakowka SG, Metzler AE, Koestner A (1982) Primary demyelination in experimental canine distemper virus-induced encephalomyelitis in gnotobiotic dogs. Sequential immunologic and morphologic findings. Acta Neuropathol (Berl) 58:1–8
Innes JRM, Saunders LZ (1962) Comparative neuropathology. Academic Press, New York London, pp 373–384
Kumpulainen T, Dahl D, Korhonen LK, Nyström HM (1983) Immunolabeling of carbonic anhydrase isoenyzme C and glial fibrillary acidic protein in paraffin-embedded tissue sections of human brain and retina. J Histochem Cytochem 31:879–886
Moulton JE (1956) Fluorescent antibody studies of demyelination in canine distemper. Proc Soc Exp Biol Med 91:460–464
Raine CS (1976) On the development of CNS lesions in natural canine distemper encephalomyelitis. J Neurol Sci 30:13–28
Robbins DS, Shirazi Y, Drysdale BE, Lieberman A, Shin HS, Shin ML (1987) Production of cytotoxic factor for oligodendrocytes by stimulated astrocytes. J Immunol 139:2593–2597
Sternberger LA, Joseph SA (1979) The unlabeled antibody method. Contrasting color staining of paired pituitary hormones without antibody removal. J Histochem Cytochem 27:1424–1431
Summers BA, Appel MJ (1987) Demyelination in canine distemper encephalomyelitis: an ultrastructural analysis. J Neurocytol 16:871–881
Vandevelde M, Zurbriggen A, Dumas M, Palmer D (1985) Canine distemper virus dose not infect oligodendrocytes in vitro. J Neurol Sci 69:133–137
Vandevelde M, Zurbriggen A, Higgins RJ, Palmer D (1985) Spread and distribution of viral antigen in nervous canine distemper. Acta Neuropathol (Berl) 67:211–218
Wisniewski H, Raine CS, Kay WJ (1972) Observations on viral demyelinating encephalomyelitis. Lab Invest 26:589–599
Zurbriggen A, Vandevelde M, Dumas M (1986) Secondary degeneration of oligodendrocytes in canine distemper virus infection in vitro. Lab Invest 54:424–431
Zurbriggen A, Vandevelde M, Dumas M, Griot C, Bollo E (1987) Oligodendroglial pathology in canine distemper virus infection in vitro. Acta Neuropathol (Berl) 74:366–373
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Supported by the Swiss National Science Foundation (grant no. 3.956.87) and the Swiss Multiple Sclerosis Society
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Mutinelli, F., Vandevelde, M., Griot, C. et al. Astrocytic infection in canine distemper virus-induced demyelination. Acta Neuropathol 77, 333–335 (1989). https://doi.org/10.1007/BF00687587
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DOI: https://doi.org/10.1007/BF00687587