Summary
Formalin-fixed, paraffin-embedded surgical specimens from 137 primary central nervous system tumors, including 26 astrocytomas (21 fibrillary, 1 protoplasmic, 1 gemistocytic and 3 pilocytic), 26 anaplastic astrocytomas, 9 glioblastomas, 1 gliosarcoma, 8 oligodendrogliomas, 4 ependymomas, 1 anaplastic ependymoma, 2 subependymomas, 3 paragangliomas, and 57 meningiomas, were immunostained with the CM1 polyclonal (pAb) and the DO-7 monoclonal (mAb) antibodies against the p53 protein, using the streptavidin/peroxidase method. In addition, two series of 17 and 9 medulloblastomas were also immunostained with the above pAb and mAb, respectively. p53 protein expression was observed in 7 fibrillary astrocytomas, 17 anaplastic astrocytomas, 5 glioblastomas, 1 gliosarcoma, 1 oligodendroglioma, 1 anaplastic ependymoma, and 4 meningiomas with the CM1 pAb. An additional 10 cases (i.e., 3 anaplastic astrocytomas and 7 meningiomas) were found to be p53 protein positive with the DO-7 mAb. Of the medulloblastomas, 8 (of the 17) and 4 (of the 9) were found to express p53 protein with CM1 pAb and DO-7 mAb, respectively. Our results indicate that p53 protein is expressed in a number of central nervous system neoplasms, and suggest that in astrocytic tumors a possible association may exist between p53 protein expression and tumor progression through increasing histological grades of malignancy.
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Albrecht S, Connelly J, Bruner JM (1992) Distribution of p53 in gliosarcomas (abstract) J Neuropathol Exp Neurol 51:363
Barbareschi M, Iuzzolino P, Pennella A, Allegranza A, Arrigoni G, Dalla Palma P, Doglioni C (1992) p53 protein expression in central nervous system neoplasms. J Clin Pathol 45:583–586
Biegel JA, Burk CD, Barr FG, Emanuel BS (1992) Evidence for a 17p tumor related locus distinct from p53 in pediatric primitive neuroectodermal tumors. Cancer Res 52:3391–3395
Bigner SH, Friedman HS, Vogelstein B, Oakes WJ, Bigner DD (1990) Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts. Cancer Res 50:2347–2350
Bruner JM, Saya H, Moser PR (1991) Immunocytochemical detection of p53 in human gliomas. Mod Pathol 4:671–674
Chung RY, Seizinger BR (1991) Tumor suppressor genes and cancer of the human nervous system. Cancer Invest 9:429–438
Dumanski JP, Carlbom E, Collins VP, Nordenskjold M, (1987) Deletion mapping of a locus on human chromosome 22 involved in the oncogenesis of menigioma. Proc Natl Acad Sci USA 84:9275–9279
El-Azouzi M, Chung RY, Farmer GE, Martuza RL, Black PMCL, Rouleau GA, Hettlich C, Hedley-Whyte ET, Zervas NT, Panagopoulos K, Nakamura Y, Gusella JF, Seizinger BR (1989) Loss of distinct regions on the short arm of chromosome 17 associated with tumorigenesis of human astrocytomas. Proc Natl Acad Sci USA 86:7186–7190
Frankel RH, Bayona W, Koslow M, Newcomb EW (1992) p53 mutations in human malignant gliomas: comparison of loss of heterozygosity with mutation frequency. Cancer Res 52:1427–1433
Friedman HS, Burger PC, Bigner SH, Trojanowski JQ, Brodeur GM, He X, Wikstrand CJ, Kurtzberg J, Berens ME, Halperin EC, Bigner DD (1988) Phenotypic and genotypic analysis of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am J Pathol 130:472–484
Fults D, Tippets RH, Thomas GA, Nakamura Y, White R (1989) Loss of heterozygosity for loci on chromosome 17p in human malignant astrocytoma. Cancer Res 49:6572–6577
Fults D, Pedone CA, Thomas GA, White R (1990) Allelotype of human malignant astrocytoma. Cancer Res 50:5784–5789
Fults D, Brockmeyer D, Tullous MW, Pedone CA, Cawthon RM (1992) p53 mutation and loss of heterozygosity on chromosomes 17 and 10 during human astrocytoma progression. Cancer Res 52:674–679
Hollstein M, Sidransky D, Vogelstein B, Harris CC (1991) p53 mutations in human cancers. Science 253:49–53
Hunter SB, Varma V (1992) Mutations in the p53 antioncogene in astrocytomas (abstract). J Neuropathol Exp Neurol 51:333
James CD, Carlbom E, Dumanski JP, Hansen M, Nordenskjold M, Collins VP, Cavenee WK (1988) Clonal genomic alterations in glioma malignancy stages. Cancer Res 48:5546–5551
James CD, Carlbom E, Nordenskjold M, Collins VP, Cavenee WK (1989) Mitotic recombination of chromosome 17 in astrocytomas. Proc Natl Acad Sci USA 86:2858–2862
James CD, He L, Carlbom E, Mikkelsen T, Ridderheim PA, Cavenee WK, Collins VP (1990) Loss of genetic information in central nervous system tumors common to children and young adults. Genes Chromosomes Cancer 2:94–102
Jaros E, Perry RH, Adam L, Kelly PJ, Crawford PJ, Kalbag RM, Mendelow AD, Sengupta RP, Pearson ADJ (1992) Prognostic implications of p53 protein, epidermal growth factor receptor, and Ki-67 labelling in brain tumors. Br J Cancer 66:373–385
Lane DP (1992) p53, guardian of the genome. Nature 358:15–16
Lane DP, Crawford LV (1979) T-antigen is bound to host protein in SV-40 transformed cells. Nature 278:261–263
Lane DP, Benchimol S (1990) p53: oncogene or antioncogene? Genes Dev 4:1–8
Levine AJ (1992) The p53 tumor suppressor gene. N Engl J Med 326:1350–1352
Levine AJ, Momand J, Finlay CA (1991) The p53 tumor suppressor gene. Nature 351:453–456
Mashiyama S, Murakami Y, Yoshimoto T, Sekiya T, Hayashi K (1991) Detection of p53 gene mutations in human brain tumors by single-strand conformation polymorphism analysis of polymerase chain reaction products. Oncogene 6:1313–1318
Mineta T, Shiraishi T, Fukuyama K, Tabuchi K (1991) Analysis of p53 expression in human glioma cell lines. Brain Tumor Pathol 8:209–211
Nigro JM, Baker SJ, Preisinger AC, Jessup JM, Hostetter R, Cleary K, Bigner SH, Davidson N, Baylin S, Devilee P, Glover T, Collins FS, Weston A, Modali R, Harris CC, Vogelstein B (1989) Mutations in the p53 gene occur in diverse human tumor types. Nature 342:705–708
Ohgaki H, Eibl RH, Wiestler OD, Yasargil MG, Newcomb EW, Kleihues P (1991) p53 mutations in nonastrocytic human brain tumors. Cancer Res 51:6202–6205
Russell DS, Rubinstein LJ (1989) Pathology of tumours of the nervous system, 5th edn. Arnold, London
Saylors RL III, Sidransky D, Frideman HS, Bigner SH, Bigner DD, Vogelstein B, Brodeur GM (1991) Infrequent p53 gene mutations in medulloblastomas. Cancer Res 51:4721–4723
Sidransky D, Mikkelsen T, Schwechheimer K, Rosenblum ML, Cavance W, Vogelstein B (1992) Clonal expansion of p53 mutant cells is associated with brain tumor progression. Nature 355:846–847
Soussi T, de Fromentel CC, May P (1990) Structural aspects of the p53 protein in relation to gene evolution. Oncogene 5:945–952
Stratton MR, Darling J, Lantos PL, Cooper CS, Reeves BR (1989) Cytogenetic abnormalities in human ependymomas. Int J Cancer 44:579–581
Varley JM, Brammar WJ, Lane DP, Swallow JE, Dolan C, Walker RA (1991) Loss of chromosome 17p13 sequences and mutation of p53 in human breast carcinomas. Oncogene 6:413–421
Vogelstein B, Kinzler KW (1992) p53 function and dysfunction. Cell 70:523–526
Wynford-Thomas D (1992) p53 in tumour pathology: can we trust immunohistochemistry? J Pathol 166:329–330
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Karamitopoulou, E., Perentes, E. & Diamantis, I. p53 protein expression in central nervous system tumors: an immunohistochemical study with CM1 polyvalent and DO-7 monoclonal antibodies. Acta Neuropathol 85, 611–616 (1993). https://doi.org/10.1007/BF00334670
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DOI: https://doi.org/10.1007/BF00334670