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The effect of ionic concentration, metabolic inhibitors and high energy phosphate compound on the microphonic potential

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Summary

Changes of the cochlear microphonic potentials were studied after replacing the perilymph by various media of different electrolyte composition, containing metabolic inhibitors and with or without energy rich phosphate compound. It was found that the ionic composition of the perilymph was not the only factor affecting the cochlear microphonic potential. Three metabolic inhibitors were used; they were 2–4 dinitrophenol, oligomycin and iodoacetic acid. To restore the microphonic potential, phosphoenolpyruvate was proved to be a useful agent.

Zusammenfassung Das Verhalten der Mikrophonpotentiale nach Austausch der Perilymphe der Scala tympani gegen Medien mit anderer Elektrolytzusammenfassung, mit Stoffwecbselblockern und mit energiereichem Phosphat wird untersucht. Dabei stellt sich heraus, daß die Elektrolytverhältnisse nicht allein ausschlaggebend sind. Durch Blockierung der Atmungskettenphosphorylierung mit DNP, durch Blockierung des ATP-Aufbaues mit Oligomycin und durch Blockierung der anaeroben Energiegewinnung mit Monojodacetat lassen sich die verschiedenen Energielieferanten in ihrer Bedeutung für die Mikrophonpotentiale isoliert austesten.

Phosphoenolpyruvat als membranpermeables energiereiches Substrat ist — nach Perfusion der Scala tympani — geeignet, den ATP-Mangel bei DNP-Vergiftung wieder auszugleichen.

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Authors and Affiliations

  1. Universitäts-Hals-Nasen-Ohrenklinik der Johann Wolfgang Goethe-Universität, Frankfurt a. Main, Germany

    J. T. -Y. Chou & K. -H. Vosteen

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  1. J. T. -Y. Chou
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  2. K. -H. Vosteen
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Chou, J.T.Y., Vosteen, K.H. The effect of ionic concentration, metabolic inhibitors and high energy phosphate compound on the microphonic potential. Arch. Klin. Exp. Ohr.-, Nas.- U. Kehlk. Heilk. 200, 300–317 (1971). https://doi.org/10.1007/BF00373312

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  • DOI: https://doi.org/10.1007/BF00373312

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