Summary
A photochemical reaction between intravenous rose bengal and xenon light was used to induce a selective thrombus in the rat anterior inferior cerebellar artery (RICA). Compound action potentials (CAPs) were recorded by electrocochleography and cochlear blood flow (CBF) was monitored by laser Doppler flow-metry. Photothrombotic occlusion of the AICA caused inner ear ischemia to various degrees with or without alterations of the CAP. With use of this model we investigated the critical range of the CBF for preserving cochlear function, represented by the CAPs induced with 8 kHz half-wave of sinusoid at 100 dB SPL. Results then showed that a CBF range between 26.7% and 42.9% of baseline was somewhat critical for maintenance of cochlear function in an acute phase of ischemia. Pretreatment with heparin significantly delayed thrombotic occlusion of the AICA in a dose-dependent manner. Further use of our model for inner ear ischemia may be useful for studying pathophysiology and pharmacological therapy of cochlear disturbances subsequent to circulatory disorders.
Similar content being viewed by others
References
Belal A (1979) The effects of vascular occlusion on the human inner ear. J Laryngol Otol 93:955–968
Dengerink HA, Axelsson A, Wright J, Miller J (1987) Histological measures of cochlear blood flow. Acta Otolaryngol (Stockh) 104:113–118
Gussen R (1976) Sudden deafness of vascular origin: a human temporal bone study. Ann Otol Rhinol Laryngol 85:94–100
Kimura RS, Perlman HB (1958) Arterial obstruction of the labyrinth. I. Cochlear changes. Ann Otol Rhinol Laryngol 67: 1–24
Kohno Y, Umemura K, Asai Y, Uematsu T, Nakashima M (1992) A new model of equilibrium dysfunction induced by photochemical damage to the inner ear microcirculation. Eur Arch Otorhinolaryngol 249:283–286
Konishi T, Butler RA, Fernandez C (1961) Effect of anoxia on cochlear potentials. J Acoust Soc Am 33:349–356
Matsuno H, Uematsu T, Nagashima S, Nakashima M (1991) Photochemically induced thrombosis model in rat femoral artery and evaluating of effects of heparin and tissue-type plasminogen activator with use of this model. J Pharmacol Methods 25:303–318
Miller J, Goodwin PC, Marks NJ (1984) Inner ear blood flow measured with a laser Doppler system. Arch Otolaryngol 110: 305–308
Perlman HB, Kimura R, Fernandez C (1959) Experiments on temporary obstruction of the internal auditory artery. Laryngoscope 69:591–613
Suga F, Preston J, Snow JB (1970) Experimental microembolization of cochlear vessels. Arch Otolaryngol 92: 213–220
Umemura K, Kohno Y, Matsuno H, Uematsu T, Nakashima M (1990) A new model for photochemically induced thrombosis in the inner ear microcirculation and the use of hearing loss as a mearsure for microcirculatory disorders. Eur Arch Otorhinolaryngol 248:105–108
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Asai, Y., Umemura, K., Kohno, Y. et al. An animal model for hearing disturbance due to inner ear ischemia: photochemically induced thrombotic occlusion of the rat anterior inferior cerebellar artery. Eur Arch Otorhinolaryngol 250, 292–296 (1993). https://doi.org/10.1007/BF00186229
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00186229