Abstract
It is shown that chromosome painting is as yet not possible for plants with very complex genomes, neither intra- nor interspecific. The reasons are inefficient blocking of dispersed repetitive sequences and insufficient signal intensity of short unique sequences. Future perspective are indicated.
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Breneman JW, Ramsey MJ, Lee DA, Eveleth GG, Minkler JL, Tucker JD (1993) The development of chromosome-specific composite DNA probes for the mouse and their application to chromosome painting. Chromosoma 102: 591–598
Cremer T, Lichter P, Borden J, Ward DC, Manuelidis L (1988) Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes. Hum Genet 80: 235–246
Dennis ES, Gerlach WL, Peacock WJ (1980) Identical polypyrimidine-polypurine satellite DNAs in wheat and barley. Heredity 44: 349–366
Fuchs J, Schubert I (1995) Localization of seed protein genes on metaphase chromosomes of Vicia faba via fluorescent in situ hybridization. Chromosome Res 3: 94–100
Fuchs J, Pich U, Meister A, Schubert I (1994) Differentiation of field bean heterochromatin by in situ hybridization with a repeated Fokl sequence. Chromosome Res 2: 25–28
Fuchs J, Brandes A, Schubert I (1995) Telomere sequence localization and karyotype evolution in higher plants. Plant Syst Evol 196: 227–241
Gerlach WL, Peacock WJ (1980) Chromosomal locations of highly repeated DNA sequences in wheat. Heredity 44: 269–276
Hoebee B, de Stoppelaar JM, Suijkerbuijk RF, Monard S (1994) Isolation of rat chromosome-specific paint probes by bivariate flow sorting followed by degenerate oligonucleotide primed-PCR. Cytogenet Cell Genet 66: 277–282
Houben A, Brandes A, Schubert I (1994) The distribution of cDNA sequences on field bean chromosomes. Genome 37: 1065–1067
Jauch A, Wienberg J, Stanyon R, Arnold N, Tofanelli S, Ishida T, Cremer T (1992) Reconstruction of genomic rearrangements in great apes and gibbons by chromosome painting. Proc Natl Acad Sci USA 89: 8611–8615
Jiang J, Gill BS (1994) Non-isotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37: 717–725
Johnson DH (1990) Molecular cloning of DNA from specific chromosomal regions by microdissection and sequence-independent amplification of DNA. Genomics 6: 243–251
Kohne DE, Levison SA, Byers MJ (1977) Room temperature method for increasing the rate of DNA reassociation by many thousandfold. The phenol emulsion reassociation technique. Biochemistry 16: 5329–5341
Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78: 6633–6637
Langford CF, Telenius H, Carter NP, Miller NGA, Tucker EM (1992) Chromosome painting using chromosome-specific probes from flow-sorted pig chromosomes. Cytogenet Cell Genet 61: 221–223
Lengauer C, Luedecke H-J, Wienberg J, Cremer T, Horsthemke B (1991) Comparative chromosome band mapping in primates by in situ suppression hybridization of band specific DNA microlibraries. Hum Evol 6: 67–71
Lichter P, Cremer T, Borden J, Manuelidis L Ward DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80: 224–234
Lucretti S, Doležel J, Schubert I, Fuchs J (1993) Flow karyotyping and sorting of Vicia faba chromosomes. Theor Appl Genet 85: 665–672
Macas J, Doležel J, Lucretti S, Pich U, Meister A, Fuchs J, Schubert I (1993) Localisation of seed protein genes on flow-sorted field bean chromosomes. Chromosome Res 1: 107–115
Marthe F, Künzel G (1994) Localization of translocation breakpoints in somatic metaphase chromosomes of barley. Theor Appl Genet 89: 240–248
Miyashita K, Vooijs MA, Tucker JD, Lee DA, Gray JW, Pallavicini MG (1994) A mouse chromosome 11 library generated from sorted chromosomes using linker-adapter polymerase chain reaction. Cytogenet Cell Genet 66: 54–57
Pich U, Houben A, Fuchs J, Meister A, Schubert I (1994) Utility of DNA amplified by degenerate oligonucleotide-primed PCR (DOP-PCR) from the total genome and defined chromosomal regions of field beans. Mol Gen Genet 243: 173–177
Pinkel D, Landegent J, Collins C, Fuscoe J, Segraves R, Lucas J, Gray J (1988) Fluorescence in situ hybridization with human chromosome-specific libraries: Detection of trisomy 21 and translocations of chromosome 4. Proc Natl Acad Sci USA 85: 9138–9142
Rosenberg C, Blakemore KJ, Kearns WG, Giraldez RA, Escallon CS, Pearson PL, Stetten G (1992) Analysis of reciprocal translocations by chromosome painting: applications and limitations of the technique. Am J Hum Genet 50: 700–705
Scherthan H, Cremer T, Arnason U, Weier H-U, Lima-de-Faria A, Frönicke L (1994) Comparative chromosome painting discloses homologous segments in distantly related mammals. Nature Genet 6: 342–347
Schubert I, Doležel J, Houben A, Scherthan H, Wanner G (1993) Refined examination of plant metaphase chromosome structure at different levels made feasible by new isolation methods. Chromosoma, 102: 96–101
Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64: 315–324
VanDevanter DR, Choongkrittaworn NM, Dyer KA, Aten J, Otto P, Behler C, Bryant EM, Rabinovitch PS (1994) Pure chromosome-specific PCR libraries from single sorted chromosomes. Proc Natl Acad Sci USA 91: 5858–5862
Vega JM, Abbo S, Feldman M, Levy AA (1994) Chromosome painting in plants: In situ hybridization with a DNA probe from a specific microdissected chromosome arm of common wheat. Proc Natl Acad Sci USA 91: 12041–12045
Weier H-UG, Polikoff D, Fawcett JJ, Greulich KM, Lee K-H, Cram S, Chapman VM, Gray JW (1994) Generation of five high-complexity painting probe libraries from flow-sorted mouse chromosomes. Genomics 21: 641–644
Wienberg J, Jauch A, Stanyon R, Cremer T (1990) Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization. Genomics 8: 347–350
Wienberg J, Stanyon R, Jauch A, Cremer T (1992) Homologies in human and Macaca fuscata chromosomes revealed by in situ hybridization with human chromosome specific DNA libraries. Chromosoma 101: 265–270
Woo S-S, Jiang J, Gill BS, Paterson AH, Wing RA (1994) Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor. Nucleic Acids Res 22: 4922–4931
Yerle M, Schmitz A, Milan D, Chaput B, Monteagudo L, Vaiman M, Frelat G, Gellin J (1993) Accurate characterization of porcine bivariate flow karyotype by PCR and fluorescence in situ hybridization. Genomics 16: 97–103
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Fuchs, J., Houben, A., Brandes, A. et al. Chromosome ‘painting’ in plants — a feasible technique?. Chromosoma 104, 315–320 (1996). https://doi.org/10.1007/BF00337219
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DOI: https://doi.org/10.1007/BF00337219