Abstract
It is controversial whether odontocetes (toothed whales) and mysticetes (whalebone whales) have a common ancestry. Cetacean karyological uniformity, which is unique among mammalian orders, suggests a monophyletic origin; however, several anatomical authorities have maintained that odontocetes and mysticetes are diphyletic. We investigated the issue using Southern blot hybridization. Two labelled restriction fragment probes from the DNA of the sei whale (a mysticete) were hybridized to restricted DNA of cetacean species representing all extant families except the Eschrichtiidae, the gray whales. The probes hybridized to specific restriction fragments in all odontocete and mysticete materials. Hybridizations showed preservation of hybridization homologies and a striking conservation of the length of highly repeated DNA sequences. The results are compatible with a common ancestry of odontocetes and mysticetes.
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References
Anbinder EM (1980) Karyology and evolution of pinnipeds, (in Russian) Nauka, Moscow, p 152
Árnason Ú (1972) The role of chromosomal rearrangement in mammalian speciation with special reference to Cetacea and Pinnipedia. Hereditas 70:112–118
Árnason Ú (1974a) Comparative chromosome studies in Cetacea. Hereditas 77:1–36
Árnason Ú (1974b) Comparative chromosome studies in Pinnipedia. Hereditas 76:179–226
Árnason Ú (1981) Localization of nucleolar organizing regions in pinniped karyotypes Hereditas 94:29–34
Árnason Ú (1982a) Karyotype stability in marine mammals. Cytogenet Cell Genet 33:274–276
Árnason Ú (1982b) Southern blot hybridization in cetaceans, using killer whale restriction fragment as a probe. Hereditas 97:47–49
Árnason Ú, Purdom IF, Jones KW (1978) Conservation and chromosomal localization of DNA satellites in balenopterid whales. Chromosoma 66:141–159
Árnason Ú, Lutley R, Sandholt B (1980) Banding studies on six killer whales: An account of C-band polymorphism and G-band patterns. Cytogenet Cell Genet 28:71–78
Árnason Ú, Purdom IF, Jones KW (1982) Cetacean molecular hybridization using balenopterid satellite DNA cRNA as probes. Hereditas 97:33–36
Duffield DA (1977) Phylokaryotypic evaluation of the Cetacea. Ph.D. Thesis, Univ of California, Los Angeles
Fay FH, Rausch VR, Feltz ET (1967) Cytogenetic comparisons of some pinnipeds (Mammalia: Eutheria). Can J Zool 45:773–778
Jarrell GH (1979) Karyotype of the bowhead whale (Balaena mysticetus). J Mammal 60:607–610
Lewin R (1982) Repeated DNA still in search of a function. Science 217:621–623
Mead JG (1975) Anatomy of the external nasal passages and facial complex in the Delphinidae (Mammalia: Cetacea). Smithson Contrib Zool 207:1–72
Nishiwaki M (1963) Taxonomical consideration on genera of Delphinidae. Sci Rep Whales Res Inst 17:93–104
Rigby PW, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:236–251
Slijper EJ (1936) Die Cetaceen, vergleichend-anatomisch und systematisch. Capita Zool 7:1–590
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Wilson AC, Bush GL, Case SM, King M-C (1975) Social structuring of mammalian populations and rates of chromosomal evolution. Proc Natl Acad Sci 72:5061–5065
Yablokov AV, Belkovich VM, Borisov VI (1972) Whales and dolphins (in Russian) Nauka, Moscow, p 472
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Árnason, Ú., Höglund, M. & Widegren, B. Conservation of highly repetitive DNA in cetaceans. Chromosoma 89, 238–242 (1984). https://doi.org/10.1007/BF00295005
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DOI: https://doi.org/10.1007/BF00295005