Summary
Sera and bloodstain extracts were subjected to isoelectric focusing in polyacrylamide gels. The focused proteins were transferred to nitrocellulose membranes by diffusion or electrophoretically, then allowed to react with specific antiserum and, after washing, with peroxidase-labeled anti-rabbit IgG. The immune complexes formed on the membranes were detected with 4-chloro-1-naphthol and hydrogen peroxide. Serum group-specific component, α2HS-glycoprotein, the sixth and the seventh component of complement, factor 13B, and plasminogen could be phenotyped with high sensitivity. Bloodstains as old as 6 months could be correctly typed for α2HS-glycoprotein by the blotting technique.
Zusammenfassung
Seren und Blutextrakte wurden auf Polyacrylamidgelen isoelektrisch fokussiert. Die fokussierten Proteine wurden auf Nitrozellulosemembranen durch Diffusion oder elektrophoretisch transferiert. Durch ein spezifisches Antiserum erfolgte auf den Membranen die AG-AK-Reaktion. Nach Waschen der Membranen wurde der gebundene Antikörper mit einem enzymisch markierten Anti-Kaninchen IgG-Antikörper nachgewiesen. Mit dieser Technik konnten die gruppenspezifische Komponente, das α2HS-Glykoprotein, die Komplementkomponenten C6 und C7, der Gerinnungsfaktor 13B und das Plasminogen sehr empfindlich phänotypisiert werden. Vorzugsweise konnten bis zu 6 Monate alte Blutspuren richtig typisiert werden.
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Tamaki, Y., Fukuda, M., Nishimukai, H. et al. Application of immunoblotting to serum protein phenotyping with reference to α2HS-glycoprotein (AHS) typing of bloodstains. Z Rechtsmed 95, 153–158 (1985). https://doi.org/10.1007/BF00201072
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DOI: https://doi.org/10.1007/BF00201072