Abstract
The aim of the present study was to map immunohistochemically the distribution of the glucocorticoid receptor (GR) in rat skin. Nuclear GR-like immunoreactivity (LI) was found in both epidermis and dermis. In the epidermis, the basal cell layer showed an intense immunoreaction; the lower part of the spinous layer was also labelled. In the dermis, the fibroblasts as well as the sweat glands, sebocytes and adipocytes were GR-immunoreactive (IR). In the root sheath of the hair follicle the staining was most intensive in the outer layer. The endothelial cells comprising the smooth muscle cells of the blood vessels, as well as the arrector pili muscle, showed GR-LI. In the peripheral nerves, the immunoreaction was localized to the nuclei of the Schwann cells and in the perineurial fibroblasts. Mast cells did not show nuclear GR-LI. Based on our immunocytochemical findings that several cell types of the skin are GR-IR, the variable physiological and pharmacological effects of glucocorticoids are easier to understand.
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References
Beato M (1989) Gene regulation by steroid hormones. Cell 56:335–344
Bruning PF, Meyer WJ, Migeon CJ (1979) Glucocorticoid receptor in cultured human skin fibroblasts. J Steroid Biochem 10:587–593
Epstein Jr EH, Munderloh NH (1981) Glucocorticoid receptors of mouse epidermis and dermis. Endocrinology 108:703–711
Goodwin P (1976) The effect of corticosteroids on cell turnover in the psoriatic patient. Br J Dermatol 94:95–100
Grunfeldt JP, Eloy L (1987) Glucocorticoids modulate vascular reactivity in the rat. Hypertension 10:608–618
Gustafsson JA, Carlstedt-Duke J, Poellinger L, Okret S, Wikström AC, Brönnegård M, Gillner M, Dong Y, Fuxe K, Cintra A (1987) Biochemistry, molecular biology, and physiology of the glucocorticoid receptor. Endocr Rev 8:185–234
Inagaki N, Miura T, Nagai H, Ono Y, Koda A (1988) Inhibition of vascular permeability increase in mice. An additional anti-allergic mechanism of glucocorticoids. Int Arch Allergy Appl Immunol 87:254–259
Laurence EB, Christophers E (1976) Selective action of hydrocortisone on postmitotic epidermal cells in vivo. J Invest Dermatol 66:222–210
Leiferman KM, Schroeter A, Kirschner MK, Spelsberg TC (1983) Characterization of the glucocorticoid receptor in human skin. J Invest Dermatol 81:355–361
Leveque JL, Pierard-Franchimont C, Rigal J de, Saint-Leger D, Pierard GE (1991) Effect of topical corticosteroids on human sebum production assessed by two different methods. Arch Dermatol Res 283:372–376
Ogawa S, Koga S, Kuwabara K, Brett J, Morrow B, Morris SA, Bilezikian JP, Silverstain SC, Stern D (1992) Hypoxia-induced increased permeability of endothelial monolayers occurs through lowering of cellular cAMP levels. Am J Physiol 262 (Cell Physiology 31):C546-C554
Okret S, Wikström A-C, Wrange Ö, Andersson B, Gustafsson J-Å (1984) Monoclonal antibodies against the rat liver glucocorticoid receptor. Proc Natl Acad Sci USA 81:1609–1613
Ponec M, Hasper I, Vianden GD, Bachra BN (1977) Effects of glucocorticosteroids on primary human skin fibroblasts. Arch Dermatol Res 259:125–134
Ponec M, Kloet ER de, Kempenaar JA (1980) Corticoids and human skin fibroblasts: intracellular specific binding in relation to growth inhibition. J Invest Dermatol 75:293–296
Rousseau GG (1984) Control of gene expression by glucocorticoid hormones. Biochem J 224:1–12
Schell H, Hornstein O, Schwartz W (1980) Human epidermal cell proliferation with regard to circadian variation of plasma cortisol. Dermatologica 161:12–21
Slaga TJ, Thompson S, Schwarz JA (1977) Binding of dexamethasone by the subcellular fractions of mouse epidermis and dermis. J Invest Dermatol 68:307–309
Stelzner TJ, O'Brien RF, Sato K, Weil JV (1988) Hypoxia-induced increased in pulmonary transvascular protein escape in rats. J Clin Invest 82:1840–1847
Walker BR, Connacher AA, Webb DJ, Edwards RW (1992) Glucocorticoids and blood pressure: a role for the cortisol/cortisone shuttle in the control of vascular tone in man. Clin Sci 83:171–178
Williams RH (1981) Textbook of endocrinology, 6th edn. Saunders, Philadelphia
Yagill Y, Koreen R, Krakoff LR (1986) Role of mineralocorticoids and glucocorticoids in blood pressure regulation in normotensive rats. Am J Physiol 251:1354–1360
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Karstila, T., Rechardt, L., Honkaniemi, J. et al. Immunocytochemical localization of glucocorticoid receptor in rat skin. Histochemistry 102, 305–309 (1994). https://doi.org/10.1007/BF00269167
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DOI: https://doi.org/10.1007/BF00269167