Abstract
In order to develop a stain with increased sensitivity and selectivity for cadmium (Cd), we synthesized and characterized 2-(8-quinolylazo)-4,5-diphenylimidazole (QAI). This chelating agent was more than twice as sensitive for Cd than the best conventional staining agents, including benzothiazolylazo-beta-naphthol. Differentiation between Cd and zinc (Zn) was achieved by immersing tissue sections in TRIS(2-aminoethyl)amine before they were stained with QAI. This pretreatment made it possible to selectively stain for Cd by blocking Zn.
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Sumi, Y., Itoh, M.T., Muraki, T. et al. Histochemical staining of cadmium with 2-(8-quinolylazo)-4,5-diphenylimidazole. Histochem Cell Biol 106, 223–227 (1996). https://doi.org/10.1007/BF02484404
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DOI: https://doi.org/10.1007/BF02484404