Summary
Acid phosphatase activity has been studied in cold microtome sections and using simultaneous azo coupling method in developing teeth and bone, and serial sections were made for the demonstrations of alkaline phosphatase.
1. In developing teeth, strongest activity of acid phosphatase was found in the distal portion of high columnar ameloblasts associated with heavy calcification in the rodent incisor, and ameloblasts and odontoblasts in adjacent occlusal surface in molar teeth. However, the activity of immatured ameloblast and crevicular aspects of molar were weaker.
2. In the epiphyseal bone trabeculae a striking acid phosphatase reaction was found.
3. As regards to the effects of decalcifying solutions to the enzymatic activity, the use of EDTA decalcifying agent (10% and pH 7 to 4) showed the best results. That is, a decrease of decalcifying time and a greater preservation of acid phosphatase activity.
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Mori, M., Takada, K. & Okamoto, J. Histochemical studies on the localization and activity of acid phosphatase in calcifying tissues. Histochemie 2, 427–434 (1962). https://doi.org/10.1007/BF00736498
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DOI: https://doi.org/10.1007/BF00736498