The correlation of individual results in laser microanalysis applied to histological slices

Summary

This work is a continuation of investigations concerning the applicability of laser analysis in histochemical techniques (Kozik et al., 1970a, b; Kozik et al., 1971a, b).Agar standards containing known amounts of Pb were subjected to laser microanalysis and optical densities of the resulting Pb spectral bands as well as volumes of the resultant craters were determined.—Based on mathematical considerations and equation has been educed by means of which the experimentally obtained photometric readings may be converted into densities corresponding to mean crater volumes. In that way it is possible to eliminate inconveniences resulting from diversity of individual crater volumes and from eventual differences in the degree of atomic excitation between samples. Using this method, the concentrations of the investigated element in histological slices become expressed in percentages.