Summary
Light and electron microscopic histochemical reactions were studied in the cells of pars intermedia of the rat. The possible correlations between enzymatic reactions and endocrine functions of these cells were discussed.
By combined formaldehyde and chloral vapour treatment the cells of the pars intermedia exhibited a strong yellow fluorescence suggesting the presence of a peptide or peptides with NH2-terminal tryptophan. Masked metachromasia after acid hydrolysis was probably due to these peptides. Only a weak or no α-glycerophosphate dehydrogenase and nonspecific esterase activity was observed in the cells of pars intermedia compared to the cells of pars distalis suggesting low production rate of hormone synthesis.
Specific and non-specific cholinesterases were demonstrated light and electron microscopically constantly in the cells bordering the lobules. These cells probably represent a certain type of glial cells. In the other cells the enzymatic activities varied markedly in intensity and distribution showing different ultrastructural localizations. Thus cholinesterase activities in the cells of pars intermedia reflect possibly different functional stages of the cells in their hormone production, storage and secretion processes.
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References
Baker, B. L., Drummond, T.: The cellular origins of corticotropin and melanotropin as revealed by immunochemical staining. Amer. J. Anat.134, 395–409 (1972)
Bridges, T. E., Fisher, A. W., Gosbee, J. L., Lederis, K., Santolaya, R. C.: Acetylcholine and cholinesterases (assays and light and electron microscopical histochemistry) in different parts of the pituitary of rat, rabbit and domestic pig. Z. Zellforsch.136, 1–18 (1973)
Böcking, A., von Deimling, O., Heitz, P., Enderlin, F.: Esterase. XV. Elektronenmikroskopische Darstellung der unspezifischen Esterase in menschlichem Nebennierenrindengewebe. Beitr. Path. Bd.153, 297–305 (1974)
Dahlström, A., Fuxe, K.: Monoamines and the pituitary gland. Acta endocr. (Kbh.)51, 301–314 (1966)
Eränkö, O., Kokko, A., Söderholm, U.: Histochemical evidence of three types of esterases in the adrenal medulla of the rat. Histochemie2, 383–388 (1962)
Hess, R., Barratt, O., Gelzer, J.: Immunofluorescent localization β-corticotropin in the rat pituitary. Experientia (Basel)24, 584–585 (1968)
Hess, R., Scarpelli, D. G., Pearse, A. G. E.: The cytochemical localization of oxidative enzymes. II. Pyridine nucleotide-linked dehydrogenases. J. biophys. biochem. Cytol.4, 753–760 (1958)
Howe, A.: The mammalian pars intermedia: A review of its structure and function. J. Endocr.59, 385–409 (1973)
Håkanson, R., Larsson, L.-I., Nobin, A., Sundler, F.: Tryptamine or tryptophyl peptides in endocrine cells of the mammalian adenohypophysis? J. Histochem. Cytochem.20, 908–916 (1972)
Håkanson, R., Sundler, F., Larsson, L.-I., Ekman, R., Sjöberg, N.-O.: Peptides with NH2-terminal tryptophan in adrenocorticotrophic hormone and melanocyte-stimulating hormone granules of the adenohypophysis. J. Histochem. Cytochem.23, 65–74 (1975)
Håkanson, R., Sundler, F., Nobin, A., Sjöberg, N.-O., Edvinson, L., Larsson, L.-I.: Peptides with NH2-terminal tryptophan in the adenohypophysis. Cell. Tiss. Res.150, 281–290 (1974)
Lewis, P. R., Shute, C. C. D.: An electron-microscopic study of cholinesterase distribution in the rat adrenal medulla. J. Microscopy89, 181–193 (1969)
Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol.39, 111–114 (1964)
Moriarty, G. C.: Adenohypophysis: Ultrastructural cytochemistry. A review. J. Histochem. Cytochem.21, 855–894 (1973)
Nakane, P. K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem.18, 9–20 (1970)
Partanen, S.: Simultaneous fluorescence histochemical demonstration of catecholamines and tryptophyl-peptides in endocrine cells. Histochemistry43, 295–303 (1975)
Partanen, S., Hervonen, A.: Monoamine-containing structures in the hypothalamo-hypophyseal system in the human fetus. Z. Anat. Entwickl.-Gesch.140, 53–60 (1973)
Pearse, A. G. E.: The esterases of the hypophysis and their functional significance. J. Path. Bact.72, 471–487 (1956)
Pearse, A. G. E.: Histochemistry, theoretical and applied, 3rd ed., vol. 2. Churchill-Livingstone, London: L. & A. Churchill 1972
Pearse, A. G. E., van Noorden, S.: The functional cytology of the human adenohypophysis. Canad. med. Ass. J.88, 462–471 (1963)
Pearse, A. G. E., Polak, J. M.: Endocrine tumours of neural crest origin: Neurolophomas, apudomas and the APUD concept. Med. Biol.52, 3–18 (1974)
Ploem, J. S.: The microscopic differentiation of the colour of formaldehyde-induced fluorescence. Progr. Brain Res.34, 27–38 (1971)
Rechardt, L.: Electron microscopic and histochemical observations on the supraoptic nucleus of normal and dehydrated rats. Acta physiol. scand. Suppl.329 (1969)
Scott, A. P., Lowry, P. J., Bennett, H. P. J., McMartin, C., Ratcliffe, J. G.: Purification and characterization of porcine corticotrophin-like intermediate lobe peptide. J. Endocr.61, 369–380 (1974a)
Scott, A. P., Lowry, P. J., Ratcliffe, J. G., Rees, L. H., Landon, J.: Corticotropin-like peptides in the rat pituitary. J. Endocr.61, 355–367 (1974b)
Solcia, E., Vasallo, G., Capella, C.: Selective staining of endocrine cells by basic dyes after acid hydrolysis. Stain Technol.43, 257–263 (1968)
Vanha-Perttula, T.: Esterases of the rat adenohypophysis. Acta physiol. scand.69, suppl. 283 (1966)
Welsch, U., Pearse, A. G. E.: Electron cytochemistry of BuChE and AChE in thyroid and parathyroid C cells, under normal and experimental conditions. Histochemie17, 1–10 (1969)
Whitaker, S., LaBella, F. S.: Electron microscopic histochemistry of cholinesterase in the posterior, intermediate and anterior lobes of the rat pituitary. Z. Zellforsch.130, 152–170 (1972)
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Bäck, N., Rechardt, L. & Partanen, S. Observations on the functional cytochemistry of pars intermedia of the rat hypophysis. Histochemistry 46, 121–130 (1976). https://doi.org/10.1007/BF02462736
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DOI: https://doi.org/10.1007/BF02462736