Summary
Immunocytochemical double staining techniques were used to study PP- and glucagon-like-immunoreactivity in pancreatic endocrine cells of mouse. An antiserum against FMRFamide appeared to react with all PP-immunoreactive endocrine cells. With fluorescence microscopy most PP/FMRFamide-immunoreactive cells also showed glucagon-immunoreactivity, but cells containing only PP-or glucagon-like substances were found as well. The proportion of cells containing PP-, glucagon, and both immunoreactivities varied strongly from islet to islet in all parts of the pancreas.
Using an electron microscopical immunogold double staining procedure on Lowicryl-embedded pancreas, PP/FMRFamide-and glucagon-immunoreactivity appeared to be present in the majority of endocrine A cells; both immunoreactivities were randomly distributed within the granules of these cells. Cells containing only PP/FMRFamide-or glucagon-immunoreactivity were also found. Glucagon-and a faint FMRFamide-immunoreactivity was also observed in osmicated epon-embedded tissue. Independent of their immunoreactivity all positive cells showed the same round electron dense secretory granules.
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Abad ME, Peeze Binkhorst FM, Elbal MT, Rombout JHWM (1987a) A comparative immunocytochemical study of the Gastro-Entero-Pancreatic (GEP) endocrine system in a stomachless and stomach-containing teleost. Gen Comp Endocrinol (in press)
Abad ME, Taverne-Thiele JJ, Rombout JHWM (1987b) Coexistence of Pancreatic Polypeptide (PP)- and glucagon-like peptides in pancreatic endocrine cells of Spartus auratus L. (Teleostei). Cell Tissue Res (in press)
Ali-Rachedi A, Varndell IM, Adrian TE, Gapp DA, Van Noorden S, Bloom SR, Polak JM (1984) Peptide YY (PYY)immunoreactivity is costored with glucagon-related immunoreactants in endocrine cells of the gut and pancreas. Histochemistry 80:487–491
Baetens D, Malaisse-Lagae F, Perrelet A, Orci L (1979) Endocrine pancreas: three dimensional reconstruction shows two types of islets of Langerhans. Science 206:1323–1325
Bell GI (1986) The glucagon superfamily: precursor structure and gene organization. Peptides 7:27–36
Bendayan M, Zollinger M (1983) Ultrastructural localization of antigenic sites on osmium fixed tissues applying protein A gold technique. J Histochem Cytochem 31:101–109
Bonner-Weir S, Bonner GC (1979) The organization of the endocrine pancreas: a hypothetical unifying view of the phylogenetic differences. Gen Comp Endocrinol 38:28–37
Bont WS (1978) The diameters of membrane vesicles fit in geometric series. J Theor Biol 74:361–375
Böttcher G, Sjölund K, Ekblad E, Håkanson R, Schwartz TW, Sundler F (1984) Coexistence of peptide YY and glicentin immunoreactivity in endocrine cells of the gut. Regul Pept 8:261–266
Böttcher G, Alumets J, Håkanson R, Sundler F (1986) Coexistence of glicentin and peptide YY in colorectal L cells in cat and man. An electron microscopic study. Regul Pept 13:283–291
Buchan AMJ (1985) Regulatory peptides in the amphibian pancreas. Can J Zool 63:2121–2124
El-Salhy M, Wilander E, Abu-Sinna G (1982) The endocrine pancreas of anuran amphibians: a histological and immunocytochemical study. Biomed Res 3:579–589
Gersell DJ, Gingerich RL, Greider MH (1979) Regional distribution and concentration of pancreatic polypeptide in the human and canine pancreas. Diabetes 28:11–15
Glover ID, Barlow DJ, Pitts JE, Wood SP, Tickle IJ, Blundell TL, Tatemoto K, Kimmel JR, Wollmer A, Strassburger W, Zhang Y-S (1985) Conformational studies on the pancreatic polypeptide hormone family. Eur J Biochem 142:379–385
Kaung HLC, Elde RP (1980) Distribution and morphometric quantitation of pancreatic endocrine cells in the frog Rana pipiens. Anat Rec 196:173–181
Leduque P, Paulin C, Dubois PM (1983) Immunocytochemical evidence of a substance related to the bovine pancreatic polypeptide-peptide YY group of peptides in the human fetal gastrointestinal tract. Regul Pept 6:219–230
Lehy T, Peranzi G, Cristina ML (1981) Correlative immunocytochemical and electron microscopic studies: identification of (entero)glucagon-, somatostatin- and pancreatic polypeptide-like-containing cells in human colon. Histochemistry 71:67–80
Orci L, Malaisse-Lagae F, Baetens D, Perrelet A (1978) Pancreatic polypeptide-rich regions in human pancreas. Lancet 2:1200–1201
Peranzi G, Lehy T (1984) Endocrine populations in the colon and rectum of cat, dog and monkey: Fine structure, immunocytochemistry and distribution. Anat Rec 210:87–100
Ravazolla M, Orci L (1980) A pancreatic polypeptide (PP)-like immunoreactant is present in the glicentin-containing cell of cat intestine. Histochemistry 67:221–224
Rombout JHWM (1977) Enteroendocrine cells in the digestive tract of Barbus conchonius (Teleostei, Cyprinidae). Cell Tissue Res 185:435–450
Rombout JHWM, Grinten CPM van der, Peeze Binkhorst FM, Taverne-Thiele JJ, Schooneveld H (1986) Immunocytochemical identification and localization of peptide hormones in the gastro-entero-pancreatic (GEP) endocrine system of mouse and a stomachless fish, Barbus conchonius. Histochemistry 84:471–483
Sjölund K, Sandén G, Hakanson R, Sundler F (1983) Endocrine cells in human intestine: an immunocytochemical study. Gastroenterology 85:1120–1130
Solcia E, Buffa R, Capella C, Fiocca R, Fontana P, Crivelli O (1979) Immunohistochemical characterization of gastro-enteropancreatic endocrine cells and related multihormonal cells problems, pitfalls and facts. In: Miyoshi A (ed) Gut peptides, secretion, function and clinical aspects. Elsevier Biomedical Press, Amsterdam, pp 303–309
Solcia E, Fiocca R, Capella C, Usellini L, Sessa F, Rindi G, Schwartz TW, Yanaihara N (1985) Glucagon- and PP-related peptides of intestinal L cells and pancreatic/gastric A or PP cells. Possible interrelationships of peptides and cells during evolution, fetal development and tumor growth. Peptides 6 (Suppl 3):223–229
Tsutsumi Y (1984) Immunohistochemical studies on glucagon, glicentin and pancreatic polypeptide in human stomach: normal and pathological conditions. Histochem J 16:869–883
Usellini L (1982) Ultrastructural localization of pancreatic polypeptide (PP)-like-immunoreactivity in the glicentin-containing cell (L cell) of human large intestine. Caryologia 35:110
Vaillant C, Taylor IL (1981) Demonstration of carboxylterminal PP-like peptides in endocrine cells and nerves. Peptides 2:31–35
Wang B-L, Larsson L-I (1985) Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electronmicroscopical double and triple staining method employing primary antibodies from the same species. Histochemistry 83:47–56
Yamamoto H, Nata K, Okamoto H (1986) Mosaic evolution of Prepropancreatic polypeptide. J Biol Chem 261:6156–6159
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Rombout, J.H.W.M., Abad, M.E., Peeze Binkhorst, F.M. et al. Coexistence of pancreatic polypeptide (PP)-and glucagon-immunoreactivity in pancreatic endocrine cells of mouse. Histochemistry 87, 1–6 (1987). https://doi.org/10.1007/BF00518717
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DOI: https://doi.org/10.1007/BF00518717