Immunogold labeling of blood-group antigens in human salivary glands using monoclonal antibodies and the streptavidin-biotin technique

Summary

We investigated the localization of blood-group antigens A, B, and H in human labial salivary and submandibular glands by applying a postembedding immunogold method using monoclonal antibodies in combination with the streptavidin-biotin bridge technique. The H, A, and B antigens were only detected in mature secretory granules (SGs), which were mainly found in cells in the late phase of the maturation cycle. In immature SGs, which were present in cells in the early or middle phases of the maturation cycle, these antigens were not detected. All other cytoplasmic organelles were not labeled by the monoclonal antibodies used. In blood-group-O secretors, H antigen was present in almost all of the mature SGs. In blood-group-A secretors, the labelling for H antigen exhibited a mosaic-like pattern, i.e. only some of the mature SGs contained H antigen. With respect to the A and B antigens, a similar mosaic-like pattern of staining was observed in blood-group-A and-B secretors, respectively. To the best of our knowledge, this is the first time that the distribution of blood-group antigens A, B, and H in human tissues has been demonstrated at the electron-microscope-level using monoclonal antibodies.