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Effect of sulfur dioxide on mucociliary activity and ciliary beat frequency in guinea pig trachea

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Summary

The effects of 30 min exposure to sulfur dioxide on mucociliary activity (MCA) and ciliary beat frequency (CBF) were studied in 31 guinea pig tracheas. MCA was measured by recording the light reflected from ciliated mucous membranes using an infrared bar code reader. CBF of single ciliated cells obtained by brushing was measured with phase-contrast microscopy. Each tracheal sample was exposed to SO2 at concentrations ranging from 2.5 to 12.5 ppm, or to air for control purposes. MCA and CBF were measured before and immediately after gas exposure. A reduction in mean MCA of 63% (P = 0.0007) and statistically insignificant changes in CBF (P > 0.05) were recorded at concentrations of 2.5 PPM SO2. Higher SO2 concentrations caused a further impairment of MCA as well as a dose-dependent decrease in CBF (P = 0.002). A concentration of 12.5 PPM SO2 induced a decrease from baseline values of approximately 80% in mean MCA and of roughly 70% in mean CBE This study demonstrates a dose-dependent SO2-induced decrease in MCA of guinea pig tracheas. The decrease in MCA was associated with an impairment of CBF only at SO2 concentrations higher than 5.0 ppm.

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Authors and Affiliations

  1. Division of Pneumology, Third Department of Internal Medicine, Johannes Gutenberg University, Mainz, Germany

    Marli Maria Knorst, Klaus Kienast, Joachim Müller-Quernheim & Rudolf Ferlinz

  2. Department of Otorhinolaryngology, Johannes Gutenberg University, Mainz, Germany

    Herbert Riechelmann

Authors
  1. Marli Maria Knorst
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  2. Klaus Kienast
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  3. Herbert Riechelmann
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  4. Joachim Müller-Quernheim
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  5. Rudolf Ferlinz
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Knorst, M.M., Kienast, K., Riechelmann, H. et al. Effect of sulfur dioxide on mucociliary activity and ciliary beat frequency in guinea pig trachea. Int. Arch Occup Environ Heath 65, 325–328 (1994). https://doi.org/10.1007/BF00405697

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  • DOI: https://doi.org/10.1007/BF00405697

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