Abstract
When isolated intact chloroplasts or cells from spinach (Spinacia oleracea L.) leaves are incubated in the light in the absence of CO2, their capacity for subsequent CO2-dependent photosynthetic oxygen evolution is drastically decreased. This inhibition is light and oxygen-dependent and can be prevented by addition of bicarbonate. It is concluded that the normal dissipation of photosynthetic energy by carbon assimilation and in processes related to photorespiration is an essential condition for the physiological stability of illuminated intact chloroplasts and cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- chl:
-
chlorophyll
References
Allen, J.F.: Effects of washing and osmotic shock on catalase activity of intact chloroplast preparations. FEBS Lett. 84, 221–224 (1977)
Asada, K., Kiso, K., Yoshikawa, K.: Univalent reduction of molecular oxygen by spinach chloroplasts on illumination. J. Biol. Chem. 249, 2175–2181 (1974)
Bahr, J.T., Jensen, R.G.: Activation of ribulose bisphosphate carboxylase in intact chloroplasts by CO2 and light. Arch. Biochem. Biophys. 185, 39–48 (1978)
Björkman, O.: Comparitive studies on photosynthesis in higher plants. In: Photophysiology, vol. VIII, pp. 1–63, Giese, A.C., ed. New York: Academic Press 1973
Cornic, G.: Effet exerée sur l'activité photosynthétique du Sinapis alba L. par une inhibition temporaire de la photorespiration se déroulant dans un air sans CO2. C.R. Acad. Sci. Paris (Sér D) 282, 1955–1958 (1976)
Egneus, H., Heber, U., Matthiesen, U., Kirk, M.: Reduction of oxygen by the electron transport chain of chloroplasts during assimilation of carbon dioxide. Biochim. Biophys. Acta 408, 252–268 (1975)
Halliwell, B.: The chloroplast at work. A review of modern developments in our understanding of chloroplast metabolism. Progr. Biophys. Mol. Biol. 33, 1–54 (1978)
Heber, U.: Stoichiometry of reduction and phosphorylation during illumination of intact chloroplasts. Biochim. Biophys. Acta 305, 140–152 (1973)
Heber, U., Santarius, K.A.: Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z. Naturforsch. 25b, 718–728 (1970)
Kirk, M., Heber, U.: Rates of synthesis and source of glycolate in intact chloroplasts. Planta 132, 131–141 (1976)
Krause, G.H., Thorne, S.W., Lorimer, G.H.: Glycolate synthesis by intact chloroplasts. Studies with inhibitors of photophosphorylation. Arch. Biochem. Biophys. 183, 471–479 (1977)
Krause, G.H., Lorimer, G.H., Heber, U., Kirk, M.R.: Photorespiratory energy dissipation in leaves and chloroplasts. In: Photosynthesis 77. Proc. Fourth Internat. Congr. on Photosynthesis, pp. 299–310, Hall, D.O., Coombs, J., Goodwin, T.W., eds. London: Biochemical Society 1978
Lorimer, G.H., Woo, K.C., Berry, J.A. and Osmond, C.B.: The C2 photorespiratory carbon oxidation cycle in leaves of higher plants: Pathway and consequences. In: Photosynthesis 77, Proc. Fourth Internat. Congr. on Photosynthesis, pp. 311–322, Hall, D.O., Coombs, J., Goodwin, T.W., eds. London: Biochemical Society 1978
Nishimura, M., Akazawa, T.: Photosynthetic activities of spinach leaf protoplasts. Plant Physiol. 55, 712–716 (1975)
Osmond, C.B., Björkman, O.: Simultaneous measurements of oxygen effects on net photosynthesis and glycolate metabolism in C3 and C4 species of Atriplex. Carnegie Institution of Washington. Yearbook 71, 141–148 (1972)
Satoh, K.: Mechanism of photoinactivation in photosynthetic systems I. The dark reaction in photoinactivation. Plant & Cell Physiol. 11, 15–27 (1970a)
Satoh, K.: Mechanism of photoinactivation in photosynthetic systems II. The occurrence and properties of two different types of photoinactivation. Plant & Cell Physiol. 11, 29–38 (1970b)
Servaites, J.C., Ogren, W.L.: Oxygen inhibition of photosynthesis and stimulation of photorespiration in soybean leaf cells. Plant Physiol., 61, 62–67 (1978)
Takahama, U., Nishimura, M.: Formation of singlet molecular oxygen in illuminated chloroplasts. Effects on photoinactivation and lipid peroxidation. Plant Cell Physiol. 16, 737–748 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Krause, G.H., Kirk, M., Heber, U. et al. O2-dependent inhibition of photosynthetic capacity in intact isolated chloroplasts and isolated cells from spinach leaves illuminated in the absence of CO2 . Planta 142, 229–233 (1978). https://doi.org/10.1007/BF00388218
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388218