Abstract
The endogenous pool of cytokinin metabolites during sexual differentiation of Mercurialis annua L. was studied with a computerized gas-chromatography-mass spectrometry system. Certain metabolites were common to both sexes: ribosides (isopentenyl-adenosine, ribosylzeatin) and the nucleotide of I6-Ade. Zeatin could be detected only in females while its nucleotide was present in males. The results were obtained with differentiating apices and whole plants. The high Z concentration and the low level of its nucleotide are related to the absence of two dominant complementary genes, determining maleness. Study of the regulation of cytokinin metabolism now seems possible.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- IPA:
-
isopentenyl adenosine
- I6-Ade:
-
isopentenyl adenine
- Z:
-
zeatin
- RZ:
-
ribosylzeatin
References
Amstrong, D.J., Burrows, W.Y., Evans, K., Skoog, F.: Isolation of cytokinins from tRNA. Biochem. Biophys. Res. Commun. 37, 451–456 (1969)
Bazin, M., Chabin, A., Durand, R.: Comparison between four isoaccepting transfer ribonucleic acids and corresponding synthetases in male and female flowers of the dioecious species Mercurialis annua L. Dev. Biol 44, 288–297 (1975)
Champault, A., Dauphin, B. Teller, G., Durand, B.; Hormonal contents, cytokinins and auxins (GC./MS./Computer systems) from three stains of Mercurialis annua, a dioecious plants. 4° Congrès de L'IAPTC Calgary 1978
Chung, S., Durand, B.: Differential cytokinin binding to dioecious plant ribosomes. FEBS Lett. 102, 211–215 (1979)
Dauphin, B., Teller, G., Durand, B.: Mise au point d'une nouvelle méthode de purification, caractérisation et dosage de cytokines endogènes, extraites de bourgeons de Mercuriales annuelles. Physiol. Vég. 15, 747–762 (1977)
Dauphin, B., Teller, G., Durand, B.: Identification and quantitative analysis of cytokinins from shoot apices of Mercurialis ambigua by gas chromatography-mass spectrometry computer system. Planta 144, 113–119 (1979)
Hall, R.H.: Cytokinins as a probe for developmental processes. Annu. Rev. Plant Physiol. 24, 415–444 (1973)
Kahlem, G., Champault, A., Louis, J.P., Bazin, M., Chabin, A., Delaigue, M., Dauphin, B., Durand, R.: Determination génétique et régulation hormonale de la différenciation sexuelle chez Mercurialis annua L. Physiol. Vég. 13, 763–779 (1975)
Kahlem, G.: Isolation and localization by histoimmunology of isoperoxydases specific for male flowers of the dioecious species Mercurialis annua L. Dev. Biol. 50, 58–67 (1976)
Louis, J.P., Durand, B.: Studies with the dioecious angiosperm Mercurialis annua L (2n=16): Correlation between genic and cytoplasmic male sterility, sex segregation and feminizing hormones (cytokinins). Mol. Gen. Genet. 165, 309–322 (1978)
Miller, C.O.: Naturally occuring cytokinis. In: Biochemistry and Physiology of Plant Growth Substances. Ottwa: Runge Press, pp. 33–45, 1968
Summons, R., Korth, J., Halpern, B., Truscott, R.J.W.: Quantitatitive cytokinin analysis using isotope dilution and Chemical ionisation mass spectrometry. Varian MAT application Note n° 36 1978
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dauphin-Guerin, B., Teller, G. & Durand, B. Different endogenous cytokinins between male and female Mercurialis annua L.. Planta 148, 124–129 (1980). https://doi.org/10.1007/BF00386412
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00386412