Abstract
Polysomes from dark-grown and illuminated barley seedlings were translated in cell-free systems. The translation products reacting with the antibody against the light-harvesting chlorophyll a/b protein (LHCP) were analyzed by polyacrylamide gel electrophoresis. It was found that, in addition to the precursor protein of LHCP, a product was obtained that co-migrated with the mature protein. Furthermore, the results show that the light-induced proly(A)RNA for LHCP is integrated into the polysomal complex without delay, indicating that the integration of LHCP into the membrane is controlled at a higher level of gene expression.
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Abbreviations
- LHCP:
-
light-harvesting chlorophyll a/b protein
References
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Müller, M., Viro, M., Balke, C. et al. Kinetics of the appearance of mRNA for light-harvesting chlorophyll a/b protein in polysomes of barley. Planta 148, 448–452 (1980). https://doi.org/10.1007/BF02395313
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DOI: https://doi.org/10.1007/BF02395313