Abstract
Polysomes from dark-grown and illuminated barley seedlings were translated in cell-free systems. The translation products reacting with the antibody against the light-harvesting chlorophyll a/b protein (LHCP) were analyzed by polyacrylamide gel electrophoresis. It was found that, in addition to the precursor protein of LHCP, a product was obtained that co-migrated with the mature protein. Furthermore, the results show that the light-induced proly(A)RNA for LHCP is integrated into the polysomal complex without delay, indicating that the integration of LHCP into the membrane is controlled at a higher level of gene expression.
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Abbreviations
- LHCP:
-
light-harvesting chlorophyll a/b protein
References
Adelman, M.R., Sabatini, D.D., Blobel, G. (1973) Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components. J. Cell Biol.56, 206–229
Apel, K. (1979) Phytochrome-induced appearance of mRNA activity for the apoprotein of the light-harvesting chlorophyll a/b protein of barley (Hordeum vulgare). Eur. J. Biochem.97, 183–189
Apel, K., Kloppstech, K. (1978) The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of the mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein. Eur. J. Biochem.85, 581–588
Armond, P.A., Staehelin, L.A., Arntzen, C.J. (1977) Spatial relationship of photosystem I, photosystem II and the light-harvesting complex in chloroplast membranes. J. Cell Biol.73, 400–418
Blobel, G., Dobberstein, B. (1975) Transfer of proteins across membranes. I Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol.67, 835–851
Boardman, N.K., Anderson, J.M., Goodchild, D.J. (1978) Chlorophyll-protein complexes and structure of mature and developing chloroplasts, In: Curr. top. bioenerg., vol. VIII, pp. 32–109, Sanadi, D.R., Vernon, L.P., eds. Academic Press, New York
Chua, N.H., Schmidt, G.W. (1978) Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc. Natl. Acad. Sci. USA75, 6110–6114
Gray, R.E., Cashmore, A.R. (1976) RNA synthesis in plant leaf tissue: the characterization of messenger RNA species lacking and containing polyadenylic acid. J. Mol. Biol.108, 595–608
Highfield, P.E., Ellis, R.J. (1978) Synthesis and transport of small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase. Nature (London)271, 420–424
Lodish, H.F. (1974) Model for the regulation of mRNA translation applied to haemoglobin synthesis. Nature (London)251, 385–388
Machold, O. (1975) On the molecular nature of chloroplast thylakoid membranes. Biochim. Biophys. Acta382, 494–505
Markwell, J.P., Thornber, J.P., Boggs, R.T. (1979) Higher plant chloroplasts. Evidence that all the chlorophyll exists as chlorophyll-protein complexes. Proc. Natl. Acad. Sci. USA76, 1233–1235
Müller, M., Viro, M., Balke, Ch., Kloppstech, K. (1979) Light-induced appearance of the mRNA for a chloroplast membrane protein in polysomes. Its in vitro translation and processing of the precursor protein. Hoppe Seyler's Z. Physiol. Chem.360, 330
Müller, M., Viro, M., Balke, Ch., Kloppstech, K. (1980) Polyadenylated mRNA for the light-harvesting chlorophyll a/b protein. Its presence in green and absence in chloroplast-free plant cells. Planta148, 444–447
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Müller, M., Viro, M., Balke, C. et al. Kinetics of the appearance of mRNA for light-harvesting chlorophyll a/b protein in polysomes of barley. Planta 148, 448–452 (1980). https://doi.org/10.1007/BF02395313
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DOI: https://doi.org/10.1007/BF02395313