Abstract
Localization of glutamine synthetase in thin sections of nitrogen-fixing Anabaena cylindrica was performed using immuno-gold/transmission electronmicroscopy. The enzyme was present in all of the three cell types possible; vegetative cells, heterocysts and akinetes. The specific gold label was always more pronounced in heterocysts compared with vegetative cells, and showed a uniform distribution in all three types. No specific label was associated with subcellular inclusions such as carboxysomes, cyanophycin granules and polyphosphate granules. When anti-glutamine synthetase antiserum was omitted, no label was observed.
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Abbreviations
- GS:
-
glutamine synthetase
References
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Bergman, B., Lindblad, P., Pettersson, A. et al. Immuno-gold localization of glutamine synthetase in a nitrogen-fixing cyanobacterium (Anabaena cylindrica). Planta 166, 329–334 (1985). https://doi.org/10.1007/BF00401169
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DOI: https://doi.org/10.1007/BF00401169