Abstract
The permeability properties of the cell membrane of a symbiotic Nostoc sp. for glutamate and aspartate were investigated. These compounds were translocated across the plasmalemma by a transport system which showed a very high affinity for glutamate and a lower one for aspartate. Since a concomitant release of glutamate was observed during the uptake of these two amino acids it is concluded that the transport proceeds via a counterexchange mechanism. In addition to this counterexchange a net release of glutamate occurred in the dark. Some aspects concerning the possible function of this transport system in the symbiotic association Geosiphon pyriforme are discussed.
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Chapman, J.S., Meeks, J.C. (1983) Glutamine and glutamate transport by Anabaena variabilis. J. Bacteriol. 156, 122–129
Essl, A. (1969) Vergleichende Untersuchungen über die Wirkungsspektren der Photophosphorylierungsprozesse und der photosynthetischen Sauerstoffproduktion bei der Blaualge Anacystis nidulans. Thesis, Würzburg
Feige, B., Gimmler, H., Jeschke, W.D., Simonis, W. (1969) Eine Methode zur dünnschichtchromatographischen Auftrennung von 14C- und 32P-markierten Stoffwechselprodukten. J. Chromatogr. 41, 80–90
Heldt, H.W. (1976) Metabolite carriers of chloroplasts. In: Encyclopedia of plant physiology, N.S., vol. 3: Transport in plants III, pp. 137–143, Stocking, C.R., Heber, U., eds. Springer, Berlin Heidelberg New York
Heldt, H.W., Rapley, L. (1970) Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate, and of dicarboxylates across the inner membrane of spinach chloroplasts. FEBS Lett. 10, 143–148
Heldt, H.W., Sauer, F., Rapley, L. (1972) Differentiation of the permeability properties of the two membranes of the chloroplast envelope. In: Proceedings of the IInd Int. Congr. on Photosynthesis, Stresa, Italy, 1971, pp. 1345–1355, Forti, G., Avron, M., Melandri, A., eds. Dr. W. Junk Publ., The Hague
Kies, L. (1984) Einzeller mit blaugrünen Endosymbionten (Cyanellen) als Objekte der Symbioseforschung und Modellorganismen für die Evolution der Chloroplasten. Biol. Rundsch. 22, 145–157
Knapp, E. (1933) Über Geosiphon pyriforme Fr. Wettst., eine intrazelluläre Pilz-Algen-Symbiose. Ber. Dtsch. Bot. Ges. 51, 210–215
Kratz, W.A., Myers, J. (1955) Nutrition and growth of several blue green algae. Am. J. Bot. 42, 282–287
Kremer, B.P., Kies, L., Rostami-Rabet, A. (1979) Photosynthetic performance of cyanelles in the endocyanomes Cyanophora, Glaucosphaera, Gloeochaete, and Glaucocystis. Z. Pflanzenphysiol. 92, 303–317
Lehner, K., Heldt, H.W. (1978) Dicarboxylate transport across the inner membrane of the chloroplast envelope. Biochim. Biophys. Acta 501, 531–544
MacKinney, G. (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322
Meeks, J.C., Enderlin, C.S., Joseph, C.M., Chapman, J.S., Lollar, M.W.L. (1985) Fixation of (13N)N2 and transfer of fixed nitrogen in the Anthoceros-Nostoc symbiotic association. Planta 164, 406–414
Michl, H. (1951) Über Papierionophorese bei Spannungsgefällen von 50 Volt/cm. Monatsh. Chem. 82, 489–493
Mollenhauer, D. (1970) Botanische Notizen Nr. 1: Beobachtungen an der Blaualge Geosiphon pyriforme. Nat. Mus. 100, 213–223
Partridge, S.M., Davis, H.F. (1950) Preferential release of aspartic acid during the hydrolysis of proteins. Nature 165, 62–63
Rai, A.N., Rowell, P., Stewart, W.D.P. (1983) Interactions between cyanobacterium and fungus during 15N2-incorporation and metabolism in the lichen Peltigera canina. Arch. Microbiol. 134, 136–142
Stewart, W.D.P., Rowell, P. (1977) Modifications of nitrogenfixing algae in lichen symbioses. Nature 265, 371–372
Strasser, P. (1982) Die Aufnahme und Abgabe von Metaboliten bei Blaualgen als Ausdruck der Anpassung an verschiedene Lebensbedingungen. Thesis, Salzburg
Trench, R.K., Pool, R.R. Jr., Logan, M., Engelland, A. (1978) Aspects of the relation between Cyanophora paradoxa (Korschikoff) and its endosymbiotic cyanelles Cyanocyta korschikoffiana (Hall & Claus). I. Growth, ultrastructure, photosynthesis and the obligate nature of the association. Proc. R. Soc. London 202, 423–443
Werdan, K., Heldt, H.W. (1972) Accumulation of bicarbonate in intact chloroplasts following a pH gradient. Biochim. Biophys. Acta 283, 430–441
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Strasser, P., Falkner, G. Characterization of the glutamate/aspartate-transport system in a symbiotic Nostoc sp.. Planta 168, 381–385 (1986). https://doi.org/10.1007/BF00392364
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DOI: https://doi.org/10.1007/BF00392364