Abstract
Recovery of photoinhibition in intact leaves of shade-grown kiwifruit was followed at temperatures between 10° and 35° C. Photoinhibition was initially induced by exposing the leaves for 240 min to a photon flux density (PFD) of 1 500 μmol·m-2·s-1 at 20° C. In additional experiments to determine the effect of extent of photoinhibition on recovery, this period of exposure was varied between 90 and 400 min. The kinetics of recovery were followed by chlorophyll fluorescence at 77K. Recovery was rapid at temperatures of 25–35° and slow or negligible below 20° C. The results reinforce those from earlier studies that indicate chilling-sensitive species are particularly susceptible to photoinhibition at low temperatures because of the low rates of recovery. At all temperatures above 15° C, recovery followed pseudo first-order kinetics. The extent of photoinhibition affected the rate constant for recovery which declined in a linear fashion at all temperatures with increased photoinhibition. However, the extent of photoinhibition had little effect on the temperature-dependency of recovery. An analysis of the fluorescence characteristics indicated that a reduction in non-radiative energy dissipation and repair of damaged reaction centres contributed about equally to the apparent recovery though biochemical studies are needed to confirm this. From an interpretation of the kinetics of photoinhibition, we suggest that recovery occurring during photoinhibition is limited by factors different from those that affect post-photoinhibition recovery.
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Abbreviations
- F o, F m, F v :
-
instantaneous, maximum, variable fluorescence
- K D, K F, K P, K T :
-
rate constants for non-radiative energy dissipation, fluorescence, photochemistry, transfer to photosystem I
- K(PI), k(R):
-
rate constants for photoinhibition and recovery
- PFD:
-
photon flux density
- PSI, II:
-
photosystem I, II
- φ i :
-
photon yield of photosynthesis (incident light)
References
Björkman, O. (1987a) High irradiance stress in higher plants and interaction with other stress factors. Progr. Photosynth. Res. 4, 11–18
Björkman, O. (1987b) Low-temperature chlorophyll fluorescence in leaves and its relationship to photon yield of photosynthesis in photoinhibition. In: Topics in photosynthesis, vol. 9: Photoinhibition, pp. 123–144, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam
Demmig, B., Björkman, O. (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. Planta 171, 171–184
Demmig, B., Winter, K., Krüger, A., Czygan, F.C. (1987) Photoinhibition and zeaxanthin formation in intact leaves. A possible role of the xanthophyll cycle in the dissipation of excess light energy. Plant Physiol. 84, 218–224
Dos Santos, C., Hall, D.O. (1982) Thylakoid polypeptides of light and dark aged chloroplasts. Plant Physiol. 70, 795–802
Greer, D.H., Berry, J.A., Björkman, O. (1986) Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery. Planta 168, 253–260
Greer, D.H., Laing, W.A., Kipnis, T. (1988) Photoinhibition of photosynthesis in intact kiwifruit (Actinidia deliciosa) leaves: effect of temperature. Planta 174, 152–158
Krause, G.H., Köster, S., Wong, S.C. (1985) Photoinhibition of photosynthesis under anaerobic conditions studied with leaves and chloroplasts of Spinacea oleracea L. Planta 165, 430–438
Kyle, D.J., Ohad, I. (1986) The mechanism of photoinhibition in higher plants and green algae. In: Encyclopedia of Plant Physiology, vol. 19: Photosynthesis III: Photosynthetic membranes and light harvesting systems. pp. 468–475, Staehelin, L.A., Arntzen, C.J., eds. Springer, Berlin
Ohad, I., Kyle, D.J., Arntzen, C.J. (1984) Membrane protein damage and repair: removal and replacement of inactivated 32-kilodalton polypeptides in chloroplast membranes. J. Cell Biol. 99, 481–485
Ögren, E., Öquist, G. (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. III. Chlorophyll fluorescence at 77K. Physiol. Plant. 62, 193–200
Ögren, E., Öquist, G., Hallgren, J.-E. (1984) Photoinhibition of photosynthesis in Lemna gibba as induced by the interaction between light and temperature. I. Photosynthesis in vivo. Physiol. Plant. 62, 181–186
Öquist, G., Greer, D.H., Ögren, E. (1987) Light stress at low temperature. In: Topics in photosynthesis, vol. 9: Photoinhibition, pp. 67–87, Kyle, D.J., Osmond, C.B., Arntzen, C.J., eds. Elsevier, Amsterdam
Nilsen, S., Chaturvedi, R., Dons, C. (1984) Photoinhibition of photosynthesis in Lemna gibba: The effect of different O2 and CO2 concentrations during photoinhibitory treatment. Acta Hort 162, 129–135
Powles, S.B. (1984) Photoinhibition of photosynthesis induced by visible light. Annu. Rev. Plant Physiol. 35, 15–44
Powles, S.B., Björkman, O. (1982) Photoinhibition of photosynthesis: effect on chlorophyll fluorescence at 77K in intact leaves and in chloroplast membranes. Planta 156, 97–107
Samuelsson, G., Lönneborg, A., Rosenqvist, E., Gustafsson, P., Öquist, G. (1985) Photoinhibition and reactivation of photosynthesis in the cyanobacterium Anacystis nidulans. Plant Physiol. 79, 992–995
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Greer, D.H., Laing, W.A. Photoinhibition of photosynthesis in intact kiwifruit (Actinidia deliciosa) leaves: Recovery and its dependence on temperature. Planta 174, 159–165 (1988). https://doi.org/10.1007/BF00394767
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DOI: https://doi.org/10.1007/BF00394767