Abstract
The spectrum of lectin binding sites as it emerges during embryonic development of Drosophila was analysed by means of fluorescein-labelled lectins. As development and morphogenesis proceed, the reaction pattern becomes more and more complex. Mannose/glucose-, mannose-, N-acetylglucosamine- and poly-N-ace-tylglucosamine-specific lectins bind ubiquitously. Nuclear envelopes only have binding sites for wheat germ agglutinin. N-acetylgalactosamine-binding lectins are specific for ectodermal derivatives. Gaβ-3-N-acetylgalac-tosamine-binding lectins are highly selective markers for neural structures, haemocytes and Garland cells. It is also shown that Drosophila laminin is differentially glycosylated. The possible implications of differential and germ layer-specific glycosylation are discussed.
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Dedicated to the memory of Jan Callaerts
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Callaerts, P., Vulsteke, V., De Loof, A. et al. Lectin binding sites during Drosophila embryogenesis. Roux's Arch Dev Biol 204, 229–243 (1995). https://doi.org/10.1007/BF00208490
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DOI: https://doi.org/10.1007/BF00208490