Summary
The development of ascending spinal pathways has been studied in the North American opossum using degeneration methods and the retrograde transport of horseradish peroxidase. Axons from caudal thoracic and/or lumbosacral levels of the spinal cord reach the lateral reticular nucleus, the inferior olivary complex, the reticular formation of the medulla and pons as well as the cerebellum very early in development. Innervation of the nucleus gracilis occurs somewhat later. Spinal axons grow into most of the caudal brain stem areas they occupy in the adult animal, including the nucleus gracilis, before there is convincing evidence that they reach the thalamus. Although spinal axons enter the cerebellum early in development their adult distribution with its characteristic discontinuities appears relatively late.
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References
Basbaum AI, Fields HL (1978) Endogenous pain control mechanisms: Review and hypothesis. Ann Neurol 4:451–462
Bauer-Moffett C, King JS (1981) The development of the inferior olivary complex in preweanling opossums. Anat Embryol 162:249–280
Cabana T, Martin GF (1981) The origin of brainstem-spinal projections at different stages of development in the North American opossum. Dev Brain Res 2:163–168
Cabana T, DiTirro F, Ho R, Martin GF (1981) The development of serotoninergic pathways within the spinal cord. Studies using the North American opossum as an experimental model. Soc for Neurosci Abst 7:181
Cutts JH, Krause WJ, Leeson CR (1978) General observations on the growth and development of the young pouch opossum, Didelphis virginiana. Biol Neonate 33:264–272
Fink RP, Heimer L (1967) Two methods for selective silver impregnation of degenerating axons and their synaptic endings in the central nervous system. Brain Res 4:369–374
Gilbert M, Stelzner D (1979) The development of descending and dorsal root connections in the lumbosacral spinal cord of the postnatal rat. J Comp Neurol 184:821–838
Grant G, Wikstein B, Berkley KJ, Aldskogius H (1982) The location of cerebellar-projecting neurons within the lumbosacral spinal cord in the cat. An anatomical study with HRP and retrograde chromatolysis. J Comp Neurol 204:336–348
Hartman CG (1952) Possums. University of Texas, Austin
Hazlett JC (1980) Development of spinocerebellar projections in the pouch young opossum. Soc for Neurosci Abst 6:640
Hazlett JC, Martin GF, Dom R (1971) Spino-cerebellar fibers of the opossum, Didelphis marsupialis virginiana. Brain Res 33:257–271
Hazlett JC, Dom R, Martin GF (1972) Spino-bulbar, spino-thalamic and medial lemniscal connections in the American opossum (Didelphis marsupialis virginiana). J Comp Neurol 146:95–118
Humbertson AO, Martin GF (1979) The development of monoaminergic brainstem-spinal systems in the North American opossum. Anat Embryol 156:301–318
Kevetter G, Yezierski RP, Chung JM, Martin RF, Willis WD (1982) Cells of origin of the spinoreticular tract in the monkey. J Comp Neurol 207:61–74
King JS, Laxson LC, Bishop GA (1982) The development of climbing fibers in the opossum. Symposium Cajal: Horizons in Neuroscience, p 56
Larsell O (1935) The development and morphology of the cerebellum in the opossum. Part I. Early development. J Comp Neurol 63:65–94
Laxson LC, King JS (1982) An electron microscopic study of the development of Purkinje cells in the cerebellar cortex of the opossum. Soc for Neurosci Abst 8:447
Leonard CM (1973) A method for assessing stages of neuronal maturation. Brain Res 53:412–416
Leonard CM (1974) Degeneration argyrophilia as an index of neuronal maturation: Studies on the optic tract of the golden hamster. J Comp Neurol 156:435–458
Leonard CM (1975) Developmental changes in olfactory bulb projections revealed by degeneration argyrophilia. J Comp Neurol 162:467–486
Martin GF, Dom R, King JS, RoBards M, Watson CRR (1975) The inferior olivary nucleus of the opossum (Didelphis marsupialis virginiana). Its organization and connections. J Comp Neurol 160:507–534
Martin GF, Andrezik JA, Crutcher K, Linauts M, Panneton M (1977) The lateral reticular nucleus of the opossum (Didelphis virginiana). II. Connections. J Comp Neurol 174:151–186
Martin GF, Beals JK, Culberson JL, Dom R, Goode G, Humbertson AO (1978) Observations on the development of brainstem-spinal systems in the North American opossum. J Comp Neurol 181:271–289
Martin GF, Cabana T, Culberson JL, Curry J, Tschismadia I (1980a) The development of corticobulbar and corticospinal systems. Studies using the North American opossum. Anat Embryol 161:197–213
Martin GF, Culberson JL, Tschismadia I (1980b) The development of major projections to the inferior olivary nucleus. Experimental studies using the North American opossum. Anat Embryol 160:187–202
Matsushita M, Hosoya Y, Ikeda M (1979) Anatomical organization of the spinocerebellar system in the cat, as studied by the retrograde transport of horseradish peroxidase. J Comp Neurol 184:81–106
Mayer DJ, Price DD (1976) Central nervous system mechanisms of analgesia. Pain 2:379–404
McCrady E (1938) The embryology of the opossum. Am Anat Memoirs, No. 16, The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania
Mehler WR (1969) Some neurological species differences, a posteriori. Ann NY Acad Sci 167:424–468
Mesulam M-M (1976) The blue reaction-product in horseradish peroxidase neurohistochemistry: incubation parameters and visibility. J Histochem Cytochem 24:1273
Mesulam M-M (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: A non-carcinogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26:106–117
Olmos JS de (1977) An improved method for the study of the central nervous connections. Exp Brain Res 29:541–551
Oswaldo-Cruz E, Rocha-Miranda CE (1968) The brain of the opossum (Didelphis marsupialis). Instituto de Biofisica, Universidade Federal do Rio de Janeiro, Rio de Janeiro pp 99
Schneider GE (1969) Two visual systems. Science 163:895–902
Shimamura M, Kogure I (1979) Reticulospinal tracts involved in the spino-bulbo-spinal reflex in cats. Brain Res 172:13–21
Shimamura M, Kogure I, Wada S (1982) Reticular neuron activities associated with locomotion in thalamic cats. Brain Res 231:51–62
Tweedle CD, Hearshen DO (1977) Ultrastructure of the developing cuneate-gracile nuclear complex in opossum. Anat Rec 187:733–734
Ulinski PS (1971) External morphology of pouch young opossum brains: A profile of opossum neurogenesis. J Comp Neurol 142:33–58
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This investigation was supported by The West Virginia University Medical Corporation, U.S.P.H.S. Grants BNS-80-08675 and 81-41335 to Dr. Martin, BNS-79-14072 to Dr. Hazlett, and NS-08798 to Dr. James King.
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Martin, G.F., Culberson, J.L. & Hazlett, J.C. Observations on the early development of ascending spinal pathways. Anat Embryol 166, 191–207 (1983). https://doi.org/10.1007/BF00305082
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DOI: https://doi.org/10.1007/BF00305082