Summary
The histochemical profile and ultrastructural properties of the bulbospongiosus muscle (BSM) fibers from 5–6 month old boars and barrows (castrated at 7 days of age), and intact week old piglets were compared. Based on myosin ATPase, preincubated at pH 4.2, BSM of boars contained predominately intermediately staining fibers, whereas BSM of barrows and piglets had a mixture of staining intensities. Fibers from boar BSM stained intensely for SDH, with subsarcolemmal and diffuse location of reaction product. Staining intensity for SDH was variable in BSM from barrows and piglets, with diffuse location of reaction product. The BSM of boars and barrows contained predominately dark fibers when stained for glycogen and phosphorylase, and the fibers were low in stored lipids. While the fibers were smaller in barrow as compared to boar BSM, ultrastructural differences between boar and barrow BSM were not detectable.
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References
Beermann DH, Cassens RG, Hausman GJ (1978) A second look at fiber type differentiation in porcine skeletal muscle. J Anim Sci 46:125–132
Bergamini E (1975) Different mechanisms in testosterone action on glycogen metabolism in rat perineal and skeletal muscles. Endocrinology 96:77–84
Breedlove SM, Arnold AP (1980) Hormone accumulation in a sexually dimorphic motor nucleus of the rat spinal cord. Science 210:564–566
Carlson BM, Herbrychová A, Gutmann E (1979) Retention of hormonal sensitivity in free grafts of the levator ani muscle. Exp Neurol 63:94–107
Colenbrander B, de Jong FH, Wensing CJG (1978) Changes in serum testosterone concentrations in the male pig during development. J Reprod Fert 53:377–380
Dubé JY, Lesage R, Tremblay RR (1976) Androgen and estrogen binding in rat skeletal and perineal muscles. Can J Biochem 54:50–55
Dubowitz V, Brooke MH (1973) Muscle biopsy: A modern approach. WB Saunders Philadelphia p 32
Eränkö O, Palkama A (1961) Improved localization of phosphorylase by use of polyvinyl pyrrolidone and high substrate concentration. J Histochem Cytochem 9:585
Galavazi G, Szirmai JA (1971) Cytomorphometry of skeletal muscle: The influence of age and testosterone on the rat M. levator ani. Z Zellforsch 121:507–530
Gutmann E (1976) Problems in differentiating trophic relationships between nerve and muscle cells. In: Thesleff S (ed) Motor innervation of muscle. Academic Press New York pp 323–343
Gutmann E, Hanzlíková V (1975) Effect of foreign innervation on contractile and histochemical properties of the transplanted levator ani muscle of the rat. Physiol Bohemoslov 24:127–136
Hanzliková V, Gutmann E (1974) The absence of androgen-sensitivity in the grafted soleus muscle innervated by the pudendal nerve. Cell Tissue Res 154:121–129
Hanzlíková V, Schiaffino S (1973) Studies on the effect of denervation in the developing muscle. III. Diversification of myofibrillar structure and origin of the heterogeneity of muscle fiber types. Z Zellforsch 147:75–85
Hart BL (1978) Reflexive mechanisms in copulatory behavior. In: McGil TE, Dewsbury DA, Sachs BD (eds) Sex and behavior. Status and prospectus. Plenum Press New York pp 205–294
Humason GL (1972) Animal tissue techniques. Third ed, WH Freeman and Company San Francisco
Karpati G, Engel WK (1967) Neuronal trophic function. Arch Neurol 17:542–545
Krieg M, Voigt KD (1977) Biochemical substrate of androgenic actions at a cellular level in prostate, bulbocavernosus/levator ani and in skeletal muscle. Acta Endocrinol (Copenh) 85 (Suppl 214):43–89
Moody WG, Enser MB, Wood JD, Restall DJ, Lister D (1978) Comparison of fat and muscle development in pietrain and large white piglets. J Anim Sci 46:618–633
Nachlas MM, Tsou K-C, de Souza E, Cheng C-S, Seligman AM (1957) Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J Histochem Cytochem 5:420–436
Pichey EL, Smith PB (1979) Denervation and developmental alterations of glycogen synthase and glycogen phosphorylase in mammalian skeletal muscle. Exp Neurol 65:118–130
Shafiq SA, Asiedu SA, Milhorat AT (1972) Effect of neonatal neurectomy on differentiation of fiber types in rat skeletal muscle. Exp Neurol 35:529–540
Suzuki A, Cassens RG (1980) A histochemical study of myofiber types in muscle of the growing pig. J Anim Sci 51:1449–1461
Turner JW, Leonard SL (1969) Glycogen phosphorylase in the rat levator ani: Activity related to testosterone, epinephrine and actinomycin D. Endocrinology 84:589–594
Venable JH (1966) Morphology of the cells of normal, testosterone-deprived and testosterone-stimulated levator ani muscles. Am J Anat 119:271–302
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Hughes, B.J., Bowen, J.M., Campion, D.R. et al. Effect of prepuberal castration on porcine bulbospongiosus muscle. Anat Embryol 168, 51–58 (1983). https://doi.org/10.1007/BF00305398
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DOI: https://doi.org/10.1007/BF00305398