Abstract
The enzymatic profiles of 109 clinical isolates ofAcinetobacter calcoaceticus subsp.anitratus andlwoffi were determined with conventional plate tests and the rapid API ZYM system (Analytab Products, Plainview, N.Y.). The majority of strains tested lacked DNase, hemolysin, protease, elastase and gelatinase. Strong enzymatic activities of butyrate esterase (C4), caprylate esterase (C8) and leucine arylamidase were detected in all isolates. No trypsin, chymotrypsin, alkaline phosphatase or glucosidase activities were present. This profile was characteristic of all isolates examined by the API ZYM system and could serve as a useful diagnostic feature ofAcinetobacter calcoaceticus subsp.anitratus and subsp.lwoffi.
Similar content being viewed by others
References
D'Amato RF, Friquez LA, Tomfohrde KM, Singerman E (1978) Rapid identification ofNeisseria gonorrhoeae andNeisseria meningitidis by using enzymatic profiles. J Clin Microbiol 7:77–81
Cordes LG, Brink EW, Checko PJ, Lentnek A, Lyons RW, Hayes PS, Wu TC, Tharr DG, Fraser DW (1981) A cluster ofAcinetobacter pneumonia in foundry workers. Annals of Int Med 95:688–693
Cowan ST (1974) Manual for the identification of medical bacteria. Second edition. Cambridge University Press. London, New York and Melbourne, pp 85–87
Donald WD, Doak WM (1967) Mimeae meningitis and sepsis. J Amer Med Assoc 200:287–289
Garcia I, Fainstein V, LeBlanc B, Bodey GP (1983) In vitro activities of newΒ-lactam antibiotics againstAcinetobacter spp. Antimicro Agents Chemother 24:297–299
Gardner DL, Pines A, Stewart Sm (1960) Fulminating and fatal pneumonia and septicaemia due toAchromobacter anitratum. Bri Med J 1:1108–1109
Hoffmann S, Mabeck CE, Vejlsgaard R (1982) Bacteriuria caused byAcinetobacter calcoaceticus biovars in a normal population and in general practice. J Clin Microbiol 16:443–451
Nolte FS, Hollick GE, Robertson RG (1982) Enzymatic activities ofLegionella pneumophila and Legionella-like organisms. J Clin Microbiol 15:175–177
Ohman DE, Cryz SJ, Iglewski BH (1980) Isolation and characterization ofPseudomonas aeruginosa PAO mutant that produces altered elastase. J Bact 142:836–842
Pal RB, Kale VV (1981)Acinetobacter calcoaceticus — an opportunistic pathogen. J Postgrad Med 27:218–221
Retailliau HF, Hightower AW, Dixon RE, Allen JR (1979)Acinetobacter calcoaceticus: A nosocomial pathogen with an unusual seasonal pattern. J Infect Dis 139:371–375
Rubin SJ, Granato PA, Wasilauskas BL (1980) Glucose (nonfermenting) gramnegative bacteria. In: Lennette EH, Balows A, Hausier Jr WJ, Truant JP (ed) Manual of Clinical Microbiology. Third edition. American Society for Microbiology. Washington, D.C., pp 263–287
Smalley DL (1982) Enzymatic profile ofPseudomonas paucimobilis. J Clin Microbiol 16:564–565
Thong ML (1975)Acinetobacter anitratus infection in man. Aust NZ J Med 5:435–439
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Poh, C.L., Loh, G.K. Enzymatic profile of clinical isolates ofAcinetobacter calcoaceticus . Med Microbiol Immunol 174, 29–33 (1985). https://doi.org/10.1007/BF02123668
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02123668