Abstract
Combining high resolution power of SDS-PAGE and IEF with the specific immunological recognition of a human antiserum directed againstEchinococcus granulosus antigens, we could identify, in 4 hydatid cystic fluids of human origin, 4 antigens with a molecular weight in the range 32-13 KD, and an antigen of 200 KD which, in reducing conditions, gave 2 bands of 67 and 52 KD. In addition, mainly in one of the cystic fluids, there were at least another 4 specific non-reducible bands with a molecular weight ranging from 80 to 40 KD. Specific parasite antigens, which constitute not more than 3% of total protein content of the cystic fluid, migrate, in isoelectric focusing, from a pH of less than 5 to more than 8.
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References
Bolton AE, Hunter WN (1973) The labelling of proteins to high specific radio-activities by conjugation to a125I-containing acrylating agent. Biochem J 133:529
Chordi A, Kagan IG (1965) Identification and characterization of antigenic components of sheep hydatid fluid by immunoelectrophoresis. J Parasitol 51:63
Cuatrecasas P (1971) Affinity chromatography. Ann Rev Biochem 40:259
King J, Laemmli UK (1971) Polypeptides of the tail fibres of bacteriophages T4. J Mol Biol 62:465
McConahey PH, Dixon FJ (1966) A method for trace iodination of proteins for immunological studies. Int Arch All Appl Immunol 29:185
Piantelli M, Pozzuoli R, Arru E, Musiani P (1977) Echinococcus granulosus: identification of subunits of the major antigens. J Immunol 119:1382
Pozzuoli R, Musiani P, Arru E, Patrono C, Piantelli M (1974) Echinococcus granulosus: evaluation of purified antigens immunoreactivity. Exp Parasitol 35:52
Switzer RC, Merril CR, Shifrin S (1979) A high sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem 98:231
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350
Wardi AH, Michos GA (1972) Alcian blue staining of glycoproteins in acrylamide disc electrophoresis. Anal Biochem 49:607
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d'Amelio, R., Pontesilli, O., Dayal, R. et al. Characterization of parasite antigens from human hydatid cyst fluid by SDS-PAGE and IEF. Med Microbiol Immunol 174, 43–50 (1985). https://doi.org/10.1007/BF02123670
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DOI: https://doi.org/10.1007/BF02123670