Skip to main content
SpringerLink
Log in

Diminished cord blood lymphocytel-selectin expression in neonatal bacterial infection

  • Neonatology
  • Published:
European Journal of Pediatrics Aims and scope Submit manuscript

Abstract

l-Selectin, a leukocyte surface glycoprotein involved in white blood cell extravasation, is rapidly down-regulated after leukocyte activation. We prospectively determined lymphocytel-selectin expression in freshly obtained cord blood samples of 98 neonates (gestational age 25–42 weeks). In eight infants with bacterial infection, the mean percentage ofl-selectinhigh lymphocytes was 32.5% (SD 20.1%), compared to 60.1% (SD 18.7%) in the control group (P<0.01). A percentage ofl-selectinhigh lymphocytes of less than 42% had a sensitivity of 75% and a specificity of 82% in identifying infected newborns. Cord blood lymphocytel-selectin expression was independent of gestational age, birth weight, umbilical artery pH, hematocrit, white blood cell count, absolute neutrophil count, C-reactive protein level, or maternal fever before delivery while there was a weak correlation with the newborn's immature/total ratio and platelet count. To our knowledge, this is the first report demonstrating downregulation of human lymphocytel-selectin expression following activation of the immune system in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CRP:

C-reactive protein

I/T:

immature/total

References

  1. Anderson DC, Abbassi O, Kishimoto TK, Koenig JM, McIntyre LV, Smith CW (1991) Diminished Lectin-, Epidermal growth factor-, Complement binding domain-cell adhesion molecule-1 on neonatal neutrophils underlies their impaired CD 18-independent adhesion to endothelial cells in vitro. J Immunol 146:3372–3379

    PubMed  Google Scholar 

  2. Benuck I, David RJ (1983) Sensitivity of published neutrophil indexes in identifying newborn infants with sepsis. J Pediatr 103:961–963

    PubMed  Google Scholar 

  3. Bevilacqua M, Butcher E, Furie B, Furie B, Gallatin M, Gimbrone M, Harlan J, Kishimoto K, Lasky L, McEver R, Paulson J, Rosen S, Seed B, Siegelman M, Springer T, Stoolman L, Tedder T, Varki A, Wagner D, Weissman I, Zimmerman G (1991) Selectins: a family of adhesion receptors. Cell 67:233

    PubMed  Google Scholar 

  4. Bührer C, Berlin C, Thiele H-G, Hamann A (1990) Lymphocyte activation and expression of the human leucocyte-endothelial cell adhesion molecule 1 (Leu-8/TQ1 antigen). Immunology 71:442–448

    PubMed  Google Scholar 

  5. Bührer C, Berlin C, Jablonski-Westrich D, Holzmann B, Thiele H-G, Hamann A (1992) Lymphocyte activation and regulation of three adhesion molecules with supposed function in homing: LECAM-1 (MEL-14 antigen), LPAM-1/2 (α4−integrin), and CD44 (Pgp-1). Scand J Immunol 35:107–120

    PubMed  Google Scholar 

  6. Corrigan JJ (1974) Thrombocytopenia: a laboratory sign of septicemia in infants and children. J Pediatr 85:219–221

    PubMed  Google Scholar 

  7. Dinarello CA (1991) Interleukin-1 and interleukin-1 antagonism. Blood 77:1627–1652

    PubMed  Google Scholar 

  8. Ganter U, Arcone R, Toniatti C, Morrone G, Ciliberto G (1989) Dual control of C-reactive protein gene expression by interleukin-1 and interleukin-6. EMBO J 8:3773–3779

    PubMed  Google Scholar 

  9. Gerdes JS, Polin RA (1987) Sepsis screen in neonates with evaluation of plasma fibronectin. Pediatr Infect Dis J 6:443–446

    PubMed  Google Scholar 

  10. Girardin E, Grau GE, Dayer J-M, Roux-Lombard P, the J5 study group, Lambert P-H (1988) Tumor necrosis factor and interleukin-1 in the serum of children with severe infectious purpura. N Engl J Med 319:397–400

    PubMed  Google Scholar 

  11. Hechtman DH, Cybulski MI, Fuchs HJ, Baker JB, Gimbrone MA (1991) Intravascular IL-8. Inhibitor of polymorphonuclear leukocyte accumulation at sites of acute inflammation. J Immunol 147:883–892

    PubMed  Google Scholar 

  12. Hendricks-Munoz KD, Shapiro DL (1990) The role of the lumbar puncture in the admission sepsis evaluation of the premature infant. J Perinatol 10:60–64

    PubMed  Google Scholar 

  13. Jung TM, Dailey MO (1990) Rapid modulation of homing receptors (gp90Mel-14) induced by activators of protein kinase C. Receptor shedding due to accelerated proteolytic cleavage at the cell surface. J Immunol 144:3130–3136

    PubMed  Google Scholar 

  14. Kanof ME, James SP (1988) Leu-8 antigen expression is diminished during cell activation but does not correlate with effector function of activated T lymphocytes. J Immunol 140:3701–3706

    PubMed  Google Scholar 

  15. Kishimoto TK, Jutila MA, Butcher EC (1990) Identification of a human peripheral lymph node homing receptor: a rapidly downregulated adhesion molecule. Proc Natl Acad Sci USA 87:2244–2248

    PubMed  Google Scholar 

  16. Manroe BL, Weinberg AG, Rosenfeld CR, Browne R (1979) The neonatal blood count in health and disease. I. Reference values for neutrophilic cells. J Pediatr 95:89–98

    PubMed  Google Scholar 

  17. Mathers NJ, Pohlandt F (1987) Diagnostic audit of C-reactive protein in neonatal infection. Eur J Pediatr 146:147–151

    PubMed  Google Scholar 

  18. Philip AGS, Hewitt JR (1980) Early diagnosis of neonatal sepsis. Pediatrics 65:1036–1041

    PubMed  Google Scholar 

  19. Reinherz EL, Morimoto C, Fitzgerald KA, Hussey RE, Daley JF, Schlossman SF (1982) Heterogeneity of human T4+ inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations. J Immunol 128:463–468

    PubMed  Google Scholar 

  20. Rodwell RL, Leslie AL, Tudehope DI (1988) Early diagnosis of neonatal sepsis using a hematologic scoring system. J Pediatr 112:761–767

    PubMed  Google Scholar 

  21. Tedder TF, Penta AC, Levine HB, Freedman AS (1990) Expression of the human leukocyte adhesion molecule, LAM1. Identity with the TQ1 and Leu-8 differentiation antigens. J Immunol 144:532–540

    PubMed  Google Scholar 

  22. Tegtmeyer FK, Horn C, Richter A, Wees J van (1992) Elastase α1 proteinase inhibitor complex, granulocyte count, ratio of immature to total granulocyte count, and C-reactive protein in neonatal septicaemia. Eur J Pediatr 151:353–356

    PubMed  Google Scholar 

  23. Töllner U (1982) Early diagnosis of septicemia in the newborn. Clinical studies and sepsis score. Eur J Pediatr 138:331–337

    PubMed  Google Scholar 

  24. Ulich TR, Castillo J del, Guo K (1989) In vivo hematologic effects of recombinant interleukin-6 on hematopoiesis and circulating numbers of RBCs and WBCs. Blood 73:108–110

    PubMed  Google Scholar 

  25. van Zee KJ, DeForge LE, Fischer E, Marano MA, Kenney JS, Remick DG, Lowry SF, Moldawer LL (1991) IL-8 in septic shock, endotoxemia, and after IL-1 administration. J Immunol 146:3478–3482

    PubMed  Google Scholar 

  26. Waage A, Brandtzaeg P, Halstensen A, Kierulf P, Espevik T (1989) The complex pattern of cytokines in serum from patients with meningococcol septic shock. Association between interleukin 6, interleukin 1, and fatal outcome. J Exp Med 169:333–338

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neonatology, Children's Hospital, 1000, Berlin 19, Germany

    C. Bührer, U. Luxenburger, B. Metze, E. Kattner & M. Obladen

  2. Department of Hematology, Children's Hospital, 1000, Berlin 19, Germany

    G. Henze

  3. Department of Obstetrics Women's Hospital, Rudolf Virchow Medical Center, Berlin Free University, 1000, Berlin 19, Germany

    J. W. Dudenhausen

Authors
  1. C. Bührer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. U. Luxenburger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Metze
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Kattner
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Henze
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. W. Dudenhausen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. Obladen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bührer, C., Luxenburger, U., Metze, B. et al. Diminished cord blood lymphocytel-selectin expression in neonatal bacterial infection. Eur J Pediatr 152, 519–522 (1993). https://doi.org/10.1007/BF01955064

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01955064

Key words

Search

Navigation