Abstract
Radiolabeled GA-17, a murine monoclonal antibody that reacts specifically with glioma cells, bound to a small-cell lung cancer (SCLC) cell line NCI-H69 derived from neural cells, both in vitro and in vivo. The affinity constant of GA-17 F (ab′)2 fragment binding to NCI-H69 was 1.02×108/M while that to the glioma cell line U87MG was 1.22×108/M. Iodine-125-labeled GA-17 F (ab′)2 fragments injected i.v. localized well in NCI-H69 cells xenografted in nude mice. The percentage of the injected dose per gram accumulated in the xenografted tumor was 6.87±1.34%g−1 (mean±SD,n=5) 24 h after injection. On the other hand, control monoclonal F (ab′)2 fragments accumulated in the xenografted tumor at 0.75±0.30%g−1. The tumor-to-blood ratio was 1.8 for NCI-H69, while that of control F (ab′)2 was 0.60. In conclusion, the radiolabeled GA-17 F (ab′)2 fragment is expected to be useful clinically to visualize the small-cell lung cancer and in radioimmunotherapy.
Similar content being viewed by others
Abbreviations
- SCLC:
-
small-cell lung cancer
- mAb:
-
monoclonal antibody
References
Abdel-Nabi HH, Schwartz AN, Higano CS, Wechter DG, Unger MW (1987) Colorectal carcinoma: detection with indium-111 anticarcinoembryonic-antigen monoclonal antibody ZCE-025. Radiology 164:617–621
Carney DN, Gazdar AF, Bepler G, Nau M, Minna JD (1985) Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res 45:2913–2920
Chatal JF, Fumoleau P, Saccavini JC, Thedrez P, Curtet C, Bianco-Arco A, Chetanneau A, Peltier P, Kremer M, Guillard Y (1987) Immunoscintigraphy of recurrences of gynecologic carcinomas. J Nucl Med 28:1807–1819
Greenwood FC, Hunter WN, Glover JS (1963) The preparation of131I labeled human growth hormone of high specific radioactivity. Biochem J 89:114–123
Hunter WN, Greenwood FC (1962) Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature 194:495–496
Kalofonos HP, Pawlikowska TR, Hemingway N, Courtenay-Lack N, Dhokia B, Snook D, Sivolapenko GB, Hooker GR, McKenzie CG, Lavender PJ, Thomas DGT, Epenetos AA (1989) Antibody guided diagnosis and therapy of brain gliomas using radiolabeled monoclonal antibodies against epidermal growth factor receptor and placental alkaline phosphatase. J Nucl Med 30:1636–1645
Kondo S, Miyatake S, Iwasaki K, Oda Y, Kikuchi H, Zu Y, Shamoto M, Namba Y (1992) Human glioma-specific antigens detected by monoclonal antibodies. J Neurosurg 30:506–511
Larson SM (1985) Radiolabeled monoclonal anti-tumor antibodies in diagnosis and therapy. J Nucl Med 26:538–545
Lindmo T, Boven E, Cuttitta F, Fedorko J, Bunn PA Jr (1984) Determination of immunoreactive fraction of radiolabeled monoclonal antibody by linear extrapolation to binding at infinite antigen excess. J Immunol Methods 72:77–89
Minna JD, Higgins GA, Glatstein EJ (1985) Cancer of the lung. In: DeVita VT Jr, Hellman S, Rosenberg SA (eds) Cancer; principle and practices of oncology, 2nd edn. Lippincott, Philadelphia, pp 507–597
Ponten J, Macintyre EH (1968) Long term culture of normal and neoplastic human glia. Acta Path Microbiol Scand 74:465–486
Saga T, Endo K, Akiyama T, Sakahara H, Mitsuru K, Watanabe Y, Nakai T, Hosono M, Yamamoto T, Toyoshima K, Konishi J (1991) Scintigraphic detection of overexpressed c-erbB-2 protooncogene products by a class-switched murine anti-c-erbB-2 protein monoclonal antibody. Cancer Res 51:990–994
Sakahara H, Endo K, Nakashima T, Koizumi M, Kunimatsu M, Kawamura Y, Ohta H, Nakamura T, Tanaka H, Kotoura Y, Yamamuro T, Hosoi S, Toyama S, Torizuka K (1987) Localization of human osteogenic sarcoma xenografts in nude mice by a monoclonal antibody labeled with radioiodine and indium-111. J Nucl Med 28:342–348
Scatchard G (1949) The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51:660–672
Wakabayashi T, Yoshida J, Seo H, Kazo K, Murata Y, Matsui N, Kageyama N (1988) Characterization of neuroectodermal antigen by a monoclonal antibody and its application in CSF diagnosis of human glioma. J Neurosurg 68:449–455
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kobayashi, H., Sakahara, H., Hosono, M. et al. Scintigraphic detection of neural-cell-derived small-cell lung cancer using glioma-specific antibody. J Cancer Res Clin Oncol 120, 259–262 (1994). https://doi.org/10.1007/BF01236381
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01236381