summary
An ependymoma was induced in the brain of a BD-IX rat by repeated doses of MNU. In vitro grown cells of the tumor were fibroblasts and stellate cells considered to be glial. Reimplantation of these cells into the brain of another BD-IX rat resulted a tumor growing in the meninges. This tumor was then repeatedly transplanted over several passages. Its gross morphology resembled that of a sarcoma; however, glial in cells could be demonstrated with silver methods. A cell line derived from the first transplanted tumor group proved to contain S-100 protein in traces in the first passages in vitro.
Explanted cells of the primary ependymoma were cloned. Two clones were established, RGL 1 and RGL 2. The cells of both clones resembled morphologically the stellate elements of the primary explant. RGL 1 contained S-100 protein. It was cloned again in the 16th subpassage. Four clones were established. Only 1 contained S-100 protein in traces. The clone RGL 1 had a subdiploid karyotype with a modal number of 39 and a large submetacentric marker. It was subcloned in the 25th passage with two clones, both having a karyotype of 41 chromosomes.
Zusammenfassung
Ein mit Methylnitrosoharnstoff erzeugter Hirntumor (Ependymom) bei einer BD-Ratte wurde in vitro kultiviert. Es wuchsen sowohl Firoblasten als auch sternförmige gliaähnliche Zellen. Reimplantation dieser Zellen in das Gehirn einer anderen BD-Ratte führte zu Tumorwachstum in den weichen Häuten. Diese Geschwulst wurde intracerebral über mehrere Passagen verimpft. Obwohl dieser Transplantationstumor morphologisch einem Sarkom ähnlich war, konnten durch Silberimprägnation Gliazellen angefärbt werden. Eine aus der ersten Transplantationsgeneration in vitro abgeleitete Zellinie enthielt S-100 Protein in Spuren.
In-vitro explantierte Zellen des mit Methylnitrosoharnstoff erzeugten Primärtumors wurden kloniert. Zwei Klone, RGL 1 und RGL 2, bestanden aus Zellen, die morphologisch den sternförmigen Elementen des Primärexplantates glichen. Der Klon RGL 1 enthielt S-100 Protein. In der 16. Passage wurde er erneut kloniert; von vier Unterklonen enthielt lediglich einer S-100 in Spuren. Dieser Klon besaß einen subdiploiden Karyotyp mit 39 Chromosomen, zusätzlich ein submetazentrisches Markerchromosom. Zwei Subklone von RGL 2 aus der 25. Passage besaßen 41 Chromosomen.
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Dedicated to Prof. Dr. K. J. Zülch on the occasion of his 65th birthday.
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Mennel, H.D., Tanaka, M. & Braun, M. In vitro growth of neoplastic rat glial cells. Z. Krebsforsch. 84, 193–202 (1975). https://doi.org/10.1007/BF00304044
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DOI: https://doi.org/10.1007/BF00304044