Summary
A medium conditioned by rat embryo cultures (RCM) promoted the adhesion of liver cells from human fetuses to plastic dish. Colonies were also formed in primary cultures of the same cells in the presence of RCM. The majority of the colonies formed were composed of large polygonal cells with a few colonies composed of both clear epithelial-like cells and fibroblast-like cells. RCM was superior to the rat embryo feeder layer for promotion of colony formation of cells. A number of colonies were formed from fetal human livers when a conditioned medium from human hepatoma cells (HCM) was used, but most of the colonies formed were composed of fibroblast-like cells. The cells derived from the polygonal cell colonies, which were formed in the presence of RCM, have been passaged four times and they are still growing with albumin-producing capacity. The effect of RCM was reduced by various physico-chemical treatments.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ballet F, Bouma M-E, Wang S-R, Amit N, Marais J, Infante R (1984) Isolation, culture and characterization of adult human hepatocytes from surgical liver biopsies. Hepatology 4:849–854
Kaighn ME, Price AM (1971) Production of albumin and other serum proteins by clonal cultures of normal human liver. Proc Natl Acad Sci USA 68:2396–2400
Lechner JF, Haugen A, Autrup H, MaClendon IA, Trump BF, Harris CC (1981) Clonal growth of epithelial cells from normal adult human bronchus. Cancer Res 41:2294–3404
LeGuilly Y, Launois B, Lenoir P, Bourel M (1973) Production of serum proteins by primary cultures of adult human livers. Biomedicine 18:248–255
McManus JFA (1946) Histological demonstration of mucin after periodic acid. Nature 1946:158–202
Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J (1982) Growth of human hepatoma cell lines with differentiated functions in chemically defined medium. Cancer Res 42:3858–3863
Peehl DM, Ham RG (1981) Clonal growth of human keratinocytes with small amounts of dialyzed serum. In Vitro Cell Dev Biol 16:526–538
Rheinwald JG (1980) Serial cultivation of human epidermal keratinocytes. In: Harris CC, Trump BF, Stoner GD (eds) Methods in cell biology, vol 21A. Academic Press, New York, pp 229–254
Scheffer WI, Keeker DJ (1980) Human liver cells in culture. In: Harris CC, Trump BF, Stoner DG (eds) Methods in cell biology, vol 21B. Academic Press, New York, pp 457–473
Sells MA, Chernoff J, Cerda A, Bowers C, Shafritz DA, Kase N, Christman JK, Acs G (1985) Long-term culture and passage of human fetal liver cells that synthesize albumin. In Vitro Cell Dev Biol 21:216–220
Stanley MA, Parkinson EK (1979) Growth requirements of human cervical epithelial cells in culture. Int J Cancer 24:407–417
Tokiwa T, Miyagiwa M, Sato J (1986) An attempt for eliminating fibroblast-like cells from fetal human liver cells in primary culture. Acta Med Okayama 40:107–111
Tokiwa T, Nakabayashi H, Miyazaki M, Sato J (1979) Isolation and characterization of diploid clones from adult and newborn rat liver cell lines. In Vitro 15:393–400
Uotila M, Ruoslahti E, Engvall E (1981) Two sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods 42:11–13
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tokiwa, T., Miyagiwa, M., Kawai, A. et al. The effect of conditioned medium on fetal human liver cells in primary culture. Res. Exp. Med. 186, 463–468 (1986). https://doi.org/10.1007/BF01852200
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01852200