Summary
In vitro assessments were carried out to study some biological aspects of immune lymphocytes producing a new lymphokine, called by the authors Toxoplasma growth inhibitory factor (Toxo-GIF), which inhibits the intracellular multiplication of Toxoplasma gondii within nonimmune mouse macrophages.
Concanavalin A and phytohemagglutinin-P were found to induce vigorous production of Toxo-GIF, whereas bacterial lipopolysaccharide did not. In vitro treatment of splenic lymphocytes with rabbit anti-mouse thymocyte serum plus complement abolished almost completely their ability to produce Toxo-GIF. Treatment of splenic lymphocytes with inhibitors of protein synthesis, cycloheximide or puromycin resulted in a remarkable reduction of the ability of sensitized lymphocytes to produce this lymphokine. Thus the production of Toxo-GIF seems to be dependent on the cellular metabolic events of sensitized T-lymphocytes.
The significant activity of Toxo-GIF was demonstrable even in the supernate of lymphocyte cultures incubated in serum-free medium and was also evident after immune lymphocytes and homologous antigen were incubated for the relatively short period of 10 h.
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References
Borges, J.S., Johnson, W.D.: Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products. J. exp. Med. 141, 483–496 (1975)
Cahall, D.L., Youmans, G.P.: Conditions for production, and some characteristics, of mycobacterial growth inhibitory factor produced by spleen cells from mice immunized with viable cells of the attenuated H37Ra strain of Mycobacterium tuberculosis. Infect. Immun. 12, 833–840 (1975)
Greene, B.M., Colley, D.G.: Eosinophils and immune mechanisms. II. Partial characterization of the lymphokine eosinophil stimulation promoter. J. Immunol. 113, 910–917 (1974)
Greene, B.M., Colley, D.G.: Eosinophils and immune mechanisms. III. Production of the lymphokine eosinophil stimulation promoter by mouse T lymphocytes. J. Immunol. 116, 1078–1083 (1976)
Hanaki, T., Sato, U., Nishimura, Y., Nobuto, K.: Studies on toxoplasmosis in domestic animals. III. Relations between T.S.C. skin-test and parasite relations on asymptomatic toxoplasmosis in swine. Jap. J. vet. Sci. 23, 509 (1961) (in Japanese)
Klun, C.L., Youmans, G.P.: The in vitro inhibition of growth of intracellular Listeria monocytogenes by lymphocyte products. Cell. Immunol. 9, 353–362 (1973)
Kolb, W.P., Granger, G.A.: Lymphocyte in vitro cytotoxicity: Characterization of mouse lymphotoxin. Cell. Immunol. 1, 122–132 (1970)
Krahenbuhl, J.L., Rosenberg, L.T., Remington, J.S.: The role of thymus-derived lymphocytes in the in vitro activation of macrophages to kill Listeria monocytogenes. J. Immunol. 111, 992–995 (1973)
Kühner, A.L., Davis, J.R.: Partial characterization of murine migration inhibitory factor (MIF). J. Immunol. 116, 140–145 (1976)
Mizoguchi, Y., Yamamoto, S., Morisawa, S.: Studies on the biosynthesis of macrophage migration inhibitory factor in delayed hypersensitivity. I. Effects of inhibitors of nucleic acid and protein synthesis on the production of macrophage migration inhibitory factor. J. Biochem. 73, 467–474 (1973)
Muraoka, S., Takeya, K., Nomoto, K.: In vitro studies on the mechanism of acquired resistance to tuberculous infection. I. The relationship between lymphocytes and macrophages in cellular immunity to tuberculous infection. Jap. J. Microbiol. 20, 115–122 (1976)
Omata, Y., Kitazawa, S., Makimura, S., Suzuki, N.: An autoimmune antibody and the antibody producing cells to the erythrocytes of rats experimentally infected with Toxoplasma gondii. Res. Bull. Obihiro Univ. 9, 57–68 (1974)
Perter, C.: Activation of mouse peritoneal cells to kill Listeria monocytogenes by T-lymphocyte products. Infect. Immun. 12, 36–41 (1975)
Scott, F.W., Csiza, C.K., Gillespie, J.H.: Feline viruses. V. Serum-neutralization test for feline panleukopenia. Cornell Vet. 60, 183–191 (1970)
Sethi, K.K., Pelster, B., Suzuki, N., Piekarski, G., Brandis, H.: Immunity to Toxoplasma gondii induced in vitro in nonimmune mouse macrophages with specifically immune lymphocytes. J. Immunol. 115, 1151–1158 (1975)
Shirahata, T., Shimizu, K., Suzuki, N.: An in vitro study on lymphocyte-mediated immunity in mice experimentally infected with Toxoplasma gondii. Jap. J. vet. Sci. 37, 235–243 (1975)
Shirahata, T., Shimizu, K., Suzuki, N.: Effects of immune lymphocyte products and serum antibody on the multiplication of Toxoplasma in murine peritoneal macrophages. Z. Parasitenk. 49, 11–23 (1976)
Tsuji, K.: Separation of lymphocytes by using the Conray 400-Ficoll method. Immunol. Cell 1, 265–268 (1971) (in Japanese)
Williams, T.W., Granger, G.A.: Lymphocyte in vitro cytotoxicity: Correlation of derepression with release of lymphotoxin from human lymphocytes. J. Immunol. 103, 170–178 (1969)
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Shirahata, T., Shimizu, K., Noda, S. et al. Studies on production of biologically active substance which inhibits the intracellular multiplication of Toxoplasma within mouse macrophages. Z. F. Parasitenkunde 53, 31–40 (1977). https://doi.org/10.1007/BF00383112
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DOI: https://doi.org/10.1007/BF00383112