Abstract
Anemia is a major clinical sign of Japanese bovine theileriosis caused byTheileria sergenti. To investigate the possible factors causing anemia in cattle, we developed a clearance test for bovine erythrocytes (Bo-RBC) in severe combined immunodeficient (SCID) mice. Clearance of Bo-RBC in the SCID mice was significantly accelerated when the mice were inoculated with a serum sample obtained from an infected calf during a highly parasitized phase but not when they were injected with a serum sample obtained during the convalescence phase. Acceleration of the clearance of Bo-RBC was also observed in mice treated with merozoite extract. Furthermore, the clearance of Bo-RBC that had been treated with merozoite extract was accelerated. A significant hemolytic activity in infected serum (highly parasitezed phase) was observed. Activities sufficient to accelerate the clearance of Bo-RBC in SCID mice and to induce in vitro hemolysis of Bo-RBC were also observed with a merozoite extract fromT. sergenti. The results suggest a possible linkage between the in vitro hemolysis of Bo-RBC and the acceleration of Bo-RBC clearance in SCID mice.
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Hagiwara, K., Tsuji, M., Ishihara, C. et al. Serum fromTheileria sergenti-infected cattle accelerates the clearance of bovine erythrocytes in SCID mice. Parasitol Res 81, 470–474 (1995). https://doi.org/10.1007/BF00931788
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DOI: https://doi.org/10.1007/BF00931788