Abstract
The human genome contains multiple copies of sequences related to the HERV-K family of endogenous retroviruses, homologous to the B-type mouse mammary tumour virus. A DNA fragment closely resembling an HERV-K long tandem repeat (LTR) was detected in a library of hncDNA clones enriched for sequences from human chromosome 19. Sites showing homology to the sequence of this fragment have been identified on human chromosome 19 by hybridization to previously mapped chromosome 19 cosmids. Thus the distribution of LTR sequences on a specific human chromosome has been mapped for the first time. We estimate the total number of such sites on human chromosome 19 to be at least 110. Many of these sites are located in the vicinity of known genes. The precise localizations (to specific cosmids) of LTR-homologous sequences on chromosome 19 can serve as a reference source and will automatically provide further insight into LTR-gene relationships as new genes are mapped onto the chromosome.
Similar content being viewed by others
References
Boerkoel CF, Kung HJ (1992) Transcriptional interaction between retroviral long terminal repeats (LTRs): mechanism of 5′ LTR suppression and 3′ LTR promoter activation of C-myc in avian B cell lymphomas. J Virol 66:4814–23
Brodsky LL Vassilyev AV, Kalaydzidis YaL, Osipov YuS, Tatuzov RL, Feramchuk SI (1992) GeneBee: the program package for biopolymer structural analysis. In: S. Gindikin (Ed.) DIMACS Series in Discrete Mathematics and Theoretical Computer Science, vol 8, pp 127–139, Princeton Univ.
Carrano AV, de Jong PJ, Branscomb E, Slezak T, Watkins BW (1989) Constructing chromosome- and region-specific cosmid maps of the human genome. Genome 31:1059–1065
Chung DW, Zhang JA, Tan CK, Davie EW, So AG, Downey KM (1991) Primary structure of the catalytic subunit of human DNA polymerase δ and chromosomal location of the gene. Proc Natl Acad Sci USA 88:11197–11201
Copeland A, Lennon G (1994) Rapid arrayed filter production using the ORCA robot. Nature 369:421–1
Feuchter AE, Freeman JD, Mager DL (1992) Strategy for detecting cellular transcripts promoted by human endogenous long terminal repeats: identification of a novel gene (CDC4L) with homology to yeast CDC4. Genomics 13:1237–1246
Feuchter-Murthy AE, Freeman JD, Mager DL (1993) Splicing of a human endogenous retrovirus to a novel phospholipase A2 related gene. Nucleic Acids Res 21:135–143
Goodchild NL, Wilkinson DA, Mager DL (1993) Recent evolutionary expansion of a subfamily of RTVL-H human endogenous retrovirus-like elements. Virology 196:778–788
Hromas R, Collins SJ, Hickstein D, Raskind W, Deaven LL, O'Hara P, Hagen FS, Kaushansky K (1991) A retinac acid-responsive human zinc finger gene, iMZF1, preferentially expressed in mycloid cells. J Biol Chem 266:14183–14187
de Jong PJ, Yokabata K, Chen C, Lohman F, Pederson L, McNinch J, Van Dilla M (1989) Human chromosome-specific partial digest libraries in lambda and cosmid vectors. Cytogen Cell Genet 51:985
Kemper RR, Ahn ER, Zhang P, Lee MY, Rabin M (1992) Human DNA polymerase δ gene maps to region 19g13.3-g13.4 by in situ hybridization. Genomics 14:205–206
Khouri G, Gruss P (1983) Enhancer elements. Cell 33:313–314
Larsson E, Kato N, Cohen M (1989) Human endogenous proviruses. Curr Top Microbiol Immunol 148:115–132
Leib-Mosch C, Brack R, Werner B, Erfle V, Hehlmann R (1990) Endogenous retroviral elements in human DNA. Cancer Res 50:5636s-5642s
Leib-Mosch C, Haltmeier M, Werner T, Geigl E-M, Brack-Werner R, Francke U, Erfle V, Hehlmann R (1993) Genomic distribution and transcription of solitary HERV-K LTRs. Genomics 18:261–269
Li W-H (1982) Evolution of duplicate genes and pseudogenes. In: Nei M, Koehn RK (eds)Evolution of genes and proteins. Sinauer Associates, Sunderland, Mass., USA, pp 14–37
Lueders K, Leder A, Leder P, Kuff E (1982) Association between a transposed α-globin pseudogene and retrovirus-like elements in the BALB/c mouse genome. Nature 295:426–428
Maeda M, Oshiman K, Tamura S, Futai M (1990) Human gastric (H+ + K+)-ATPase gene. Similarity to (Na+ + K+)-ATPase genes in exon/intron organization but difference in control region. J Biol Chem 256:9027–9032
Obradovic D, Borodin AM, Kopantsev EP, Wagner LL, Volik SV, Ermolayeva OD, Lebedev YuB, Monastyrskaya GS, Sverdlov ED (1994) The construction and characterization of an arrayed library of human chromosome 19 transcribed sequences from human-hamster hybrid cells. Bioorg Khimia 20:919–930
Olsen AS, Combs J, Garcia E, Elliott J, Amemiya C, de Jong P, Threadgill G (1993) Automated production of high density cosmid and YAC colony filters using a robotic workstation. Biotechniques 14:116–123
Overhauser J, Mewar R, Rojas K, Lia K, Kline AD, Silverman GA (1993) STS map of genes and anonymous DNA fragments on human chromosome 18 using a panel of somatic cell hybrids. Genomics 15:387–391
Scheidereit C, Beato M (1984) Contacts between hormone receptor and DNA double helix within a glucocorticoid regulatory element of mouse mammary tumor virus. Proc Natl Acad Sci USA 80:3029–3033
Song I, Yamada T, Trent JM (1992) Mapping of the gene encoding the a-subunit of the human H+, K+-ATPase to chromosome 19q13.1 by fluorescent in situ hybridization. Genomics 14:547–548
Sugino H, Oshimura M, Matsubara K (1992) Banding profiles of LTR of human endogenous retrovirus HERV-A in 24 chromosomes in somatic cell hybrids. Genomics 13:461–464
Suzuki H, Hosokawa Y, Toda H, Nishikimi M, Ozawa T (1990) Common protein-binding sites in the 5′-flanking regions of human genes for cytochrome c1 and ubiquinone binding protein. J Biol Chem 265:8159–8163
Ting CN, Rosenberg MP, Snow CM, Samuelson LC, Meisler MH (1992) Endogenous retroviral sequences are required for tissuespecific expression of a human salivary amylase gene. Genes Dev 6:1457–1465
Vogetseder W, Dumfahrt A, Mayersbach P, Schonitzer D, Dierich MP (1993) Antibodies in human sera recognizing a recombinant outer membrane protein encoded by the envelope gene of the human endogenous retrovirus K. AIDS Res Hum Retrovir 9:687–694
Author information
Authors and Affiliations
Additional information
Communicated by G. Georgieo
Rights and permissions
About this article
Cite this article
Lebedev, Y.B., Volik, S.V., Obradovic, D. et al. Physical mapping of sequences homologous to an endogenous retrovirus LTR on human chromosome 19. Molec. Gen. Genet. 247, 742–748 (1995). https://doi.org/10.1007/BF00290406
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00290406