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Automatic chromosome processing

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Summary

A method is described for the automatic processing of metaphase chromosomes in skin fibroblast and amniotic fluid epithelial cells growing directly on microscope slides using a commercially available staining machine. The same apparatus is used also for the staining of chromosome preparations from all sources and for smears to demonstrate X chromatin using acetic orcein. A brief description of a trypsin banding method with exposure times suitable for incorporation in an automatic programme is also included.

Zusammenfassung

Es wird eine automatische Methode zur Analyse von Metaphase-chromosomen aus Hautfibroblasten und Amnionzellen nach direkter Kultur der Zellen auf Objektträgern mit Hilfe einer im Handel erhältlichen Färbeapparatur beschrieben. Der gleiche Apparat kann auch für die Färbung von Chromosomen anderer Herkunft und für die Färbung von X-Chromatin mit saurem Orcein aus Zellabstrichen benutzt werden. Kurz beschrieben wird eine Trypsinbandenmethode, deren Inkubationszeiten die Anwendung in einem automatischen Programm erlauben.

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References

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Authors and Affiliations

  1. Department of Cytogenetics, Queen Elizabeth Hospital for Children, Hackney Road, E2 8PS, London

    L. J. Butler, Sylvia Briddon & Edna L. Jackson

Authors
  1. L. J. Butler
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  2. Sylvia Briddon
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  3. Edna L. Jackson
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Butler, L.J., Briddon, S. & Jackson, E.L. Automatic chromosome processing. Hum Genet 22, 229–232 (1974). https://doi.org/10.1007/BF00284826

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  • DOI: https://doi.org/10.1007/BF00284826

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