Summary
While it has been shown previously (Zenzes et al., 1978; Ohno et al., 1978) that when dissociated testicular cells are exposed to anti-H-Y antiserum in vitro they are prevented from reorganizing into testicular structures, forming ovarian follicular structures instead, the most conclusive evidence for the action of H-Y antigen would be the conversion of ovarian cells into testicular organization. Testing for H-Y antigen of the medium collected from cultivated testicular cells revealed a positive reaction. Dissociated ovarian cells of newborn rats cultivated in this medium reorganize into testicular structures. It is concluded that H-Y antigen is responsible for this histomorphologic change.
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Zenzes, M.T., Wolf, U. & Engel, W. Organization in vitro of ovarian cells into testicular structures. Hum Genet 44, 333–338 (1978). https://doi.org/10.1007/BF00394298
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DOI: https://doi.org/10.1007/BF00394298