Summary
Sympathetic chain ganglia of newborn rats were cultured in Rose chambers with or without hydrocortisone. After one week, the cultures were examined by light microscopy for formaldehyde-induced catecholamine fluorescence and by electron microscopy after fixation in 5% glutaraldehyde solution and thereafter in 1% osmium tetroxide. Hydrocortisone (10 mg/l) caused a great increase in the number of the small, intensely fluorescent (SIF) cells in the ganglion explants, and the fluorescence intensity of these cells was also increased. The SIF cells corresponded to small, granule-containing (SGC) cells in the electronmicros copic preparations, and in addition to an increase in their number there was also an increase in the size and number of granular vesicles in the presence of hydrocortisone. In control cultures the granular vesicles were round (about 100 nm in diameter) or elongated (40–150 nm in cross section and 150–250 nm in length); both types of vesicles contained electron dense cores. In hydrocortisone-containing cultures round granular vesicles up to 200 nm in diameter were also observed; the cores of these vesicles were of variable electron density. It is concluded that in tissue culture, hydrocortisone causes an increased formation of catecholamine-containing granular vesicles in SIF-SGC cells and their precursors and an increase in the number of these cells.
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Björklund, A., Cegrell, L., Falck, B., Ritzen, M., Rosengren, E.: Dopamine-containing cells in sympathetic ganglia. Acta physiol. scand. 78, 334–338 (1970).
Chamley, J.H., Mark, G.E., Burnstock, G.: Sympathetic ganglia in culture. II. Accessory cells. Z. Zellforsch. (in press) (1972b).
Chamley, J. H., Mark, G. E., Campbell, G. R., Burnstock, G.: Sympathetic ganglia in culture. I. Neurons. Z. Zellforsch. (in press) (1972a).
Coupland, R.E., Hopwood, D.: The mechanism of the differential staining reaction for adrenaline- and noradrenaline-storing granules in tissues fixed in glutaraldehyde. J. Anat. (Lond.) 100, 227–243 (1966).
Coupland, R.E., MacDougall, J.D.B.: Adrenaline formation in noradrenaline-storing chromaffin cells in vitro induced by corticosterone. J. Endocr. 36, 317–324 (1966).
Csillik, B., Kalman, G., Knyihar, E.: Adrenergic nerve endings in the feline cervicale superius ganglion. Experientia (Basel) 23, 477–478 (1967).
Elfvin, L. G.: A new granule-containing nerve cell in the inferior mesenteric ganglion of the rabbit. J. Ultrastructure. Res. 22, 37–44 (1968).
Eränkö, L.: Ultrastructure of the developing sympathetic nerve cell and the storage of catecholamines. Brain Res. (in press) 1972.
Eränkö, L., Eränkö, O.: Effect of hydrocortisone on histochemically demonstrable catecholamines in the sympathetic ganglia and extra-adrenal chromaffin tissue of the rat. Acta physiol. scand. 84, 125–133 (1972).
Eränkö, O.: The practical histochemical demonstration of catecholamines by formaldehyde-induced fluorescence. J. roy. micr. Soc. 87, 259–276 (1967).
Eränkö, O., Eränkö, L.: Small intensely fluorescent granule-containing cells in the sympathetic ganglion of the rat. Progr. Brain Res. 34, 39–51 (1971).
Eränkö, O., Eränkö, L., Hill, C. E., Burnstock, G.: Hydrocortisone-induced increase in the number of small intensely fluorescent cells and their histochemically demonstrable catecholamine content in cultures of sympathetic ganglia of the newborn rat. Histochem. J. 4, 49–58 (1972a).
Eränkö, O., Härkönen, M.: Histochemical demonstration of fluorogenic amines in the cytoplasm of sympathetic ganglion cells of the rat. Acta physiol. scand. 58, 285–286 (1963).
Eränkö, O., Härkönen, M.: Effect of axon division on the distribution of noradrenaline and acetylcholinesterase in sympathetic neurons of the rat. Acta physiol. scand. 63, 411–412 (1965a).
Eränkö, O., Härkönen, M.: Monoamine-containing small cells in the superior cervical ganglion of the rat and an organ composed of them. Acta physiol. scand. 63, 511–512 (1965b).
Eränkö, O., Heath, J. W., Eränkö, L.: Effect of hydrocortisone on the ultrastructure of the small, granule-containing cells in the superior cervical ganglion of the newborn rat. (In the course of publication) (1972b).
Eränkö, O., Lempinen, M., Räisänen, L.: Adrenaline and noradrenaline in the organ of Zuckerkandl and adrenals of newborn rats treated with hydrocortisone. Acta physiol. scand. 66, 253–254 (1966).
Grillo, M. A.: Electron microscopy of sympathetic tissues. Pharmacol. Rev. 18, 387–399 (1966).
Hökfelt, T.: Distribution of noradrenaline storing particles in peripheral adrenergic neurons as revealed by electron microscopy. Acta physiol. scand. 76, 427–440 (1969).
Jacobowitz, D.: Histochemical studies of the relationship of chromaffin cells and adrenergic nerve fibres of the cardiac ganglia of several species. J. Pharmacol. exp. Ther. 158, 227–240 (1967).
Jacobowitz, D.: Catecholamine fluorescence studies of adrenergic neurons and chromaffin cells in sympathetic ganglia. Fed. Proc. 29, 6, 1929–1944 (1970).
Kanerva, L.: The postnatal development of monoamines and cholinesterase in the paracervical ganglion of the rat uterus. Progr. Brain Res. 34, 433–444 (1971).
Kanerva, L.: Ultrastructure of sympathetic ganglion cells and granule-containing cells in the paracervical (Frankenhäuser) ganglion of the newborn rat. Z. Zellforsch. 126, 25–40 (1972).
Kanerva, L., Täräväinen, H.: Electron microscopy of the paracervical (Frankenhäuser) ganglion of the adult rat. Z. Zellforsch. 129, 161–177 (1972).
Lempinen, M.: Extra-adrenal chromaffin tissue of the rat and the effect of cortical hormones on it. Acta physiol. scand. 62, Suppl. 231 (1964).
Lever, J. D., Presley, R.: Studies on the sympathetic neurone in vitro. Progr. Brain Res. 34, 499–512 (1971).
Mascorro, J. A., Yates, R. D.: Microscopic observations in abdominal sympathetic paraganglia. Tex. Rep. Biol. Med. 28, 59–68 (1970).
Matthews, M. R., Raisman, G.: The ultrastructure and somatic efferent synapses of small granule-containing cells in the superior cervical ganglion. J. Anat. (Lond.) 105, 255–282 (1969).
Norberg, K. A., Hamberger, B.: The sympathetic adrenergic neuron. Acta physiol. scand. 63, Suppl. 238 (1964).
Norberg, K. A., Ritzén, M., Ungerstedt, U.: Histochemical studies on a special catecholamine-containing cell type in sympathetic ganglia. Acta physiol. scand. 67, 260–270 (1966).
Norberg, K. A., Sjöqvist, F.: New possibilities for adrenergic modulation of ganglionic transmission. Pharmacol. Rev. 18, 743–751 (1966).
Olson, L.: Outgrowth of sympathetic adrenergic neurons in mice treated with a nerve growth factor (NGF). Z. Zellforsch. 81, 155–173 (1967).
Orden, L. S. III van, Burke, J. P., Geyer, M., Lodoen, F. V.: Localization of depletion-sensitive and depletion-resistant norepinephrine sites in autonomic ganglia. J. Pharmacol. exp. Ther. 174, 56–71 (1970).
Owman, C., Sjöstrand, N.-O.: Short adrenergic neurons and catecholamine containing cells in vas deferens and accessory male genital glands of different mammals. Z. Zellforsch. 66 300–320 (1965).
Rose, G. C.: A separable and multipurpose tissue culture chamber. Tex. Rep. Biol. Med. 12, 1074–1083 (1954).
Salk, E. S., Youngner, J. S., Ward, E. N.: Use of color change of phenol red as the indicator in titrating poliomyelitis virus on its antibody in a tissue culture system. Appendix. Method of preparing medium 199. Amer. J. Hyg. 60, 214–230 (1954).
Siegrist, G., Dolivo, M., Dunant, Y., Foroglou-Kerameus, C., Ribaupierre, F. de, Rouiller, C.: Ultrastructure and function of the chromaffin cells in the superior cervical ganglion of the rat. J. Ultrastruct. Res. 25, 381–407 (1968).
Taxi, J., Gautron, J., L'Hermite, P.: Données ultrastructurales sur une éventuelle modulation adrénergique de l'activé du ganglion cervical superieur du Rat. C. R. Acad. Sci. (Paris) 269, 1281–1284 (1969).
Watanabe, H.: Adrenergic nerve endings in the peripheral autonomic ganglion. Experientia (Basel) 26, 69–70 (1970).
Watanabe, H.: Adrenergic nerve elements in the hypogastric ganglion of the guinea-pig. Amer. J. Anat. 130, 305–330 (1971).
Williams, T. H.: Electron microscopic evidence for an autonomic interneuron. Nature (Lond.), 214, 39–310 (1967).
Williams, T. H., Palay, S. L.: Ultrastructure of the small neurons in the superior cervical ganglion. Brain Res. 15, 17–34 (1969).
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This work was supported by grants from the National Heart Foundation, the Australian Research Grants Committee and the Sigrid Juselius Foundation.
University of Melbourne Senior Research Fellow, September, 1971 – August, 1972; present address: Department of Anatomy, University of Helsinki, Siltavuorenpenger, Helsinki, Finland, 00170.
Holder of a grant from the National Health and Medical Research Council of Australia.
Sunshine Foundation and Rowden White Research Fellow in the University of Melbourne, September, 1971 – August, 1972; present address: Department of Anatomy, University of Helsinki, Siltavuorenpenger, Helsinki, Finland, 00170.
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Eränkö, O., Heath, J. & Eränkö, L. Effect of hydrocortisone on the ultrastructure of the small, intensely fluorescent, granule-containing cells in cultures of sympathetic ganglia of newborn rats. Z.Zellforsch 134, 297–310 (1972). https://doi.org/10.1007/BF00307167
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DOI: https://doi.org/10.1007/BF00307167