Summary
After exposure of mouse cecal mucosa to lanthanum during primary fixation in glutaraldehyde, the metal was found as discrete particles covering microvilli and microorganisms, as a continuous layer outlining the lateral surfaces of epithelial cells, and as particles bound to collagen fibrils. The results suggest that lanthanum acts primarily as a stain of the surfaces of certain cells and extracellular structures, and to a lesser extent as an inert probe of the extracellular space.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Doggenweiler, C. F., Frenk, S.: Staining properties of lanthanum on cell membranes. Proc. nat. Acad. Sci., (Wash.) 53, 425–430 (1965)
Fahimi, H. D., Cotran, R. S.: Permeability studies in heat-induced injury of skeletal muscle using lanthanum as fine structural tracer. Amer. J. Path. 62, 143–158 (1971)
Henrikson, R. C.: Ultrastructural aspects of mouse cecal epithelium. Z. Zellforsch. 140, 445–449 (1973)
Kellenberger, E., Ryter, A., Séchaud, J.: Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. biophys. biochem. Cytol. 4, 671–678 (1958)
Revel, J. P., Karnovsky, M. J.: Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33, C7-C12 (1967)
Stoeckenius, W.: Electron microscopy of DNA molecules “stained” with heavy metal salts. J. biophys. biochem. Cytol. 11, 297–310 (1961)
Author information
Authors and Affiliations
Additional information
This research was supported by NIH Grants GM-15289 and NS-01538-16.
Rights and permissions
About this article
Cite this article
Henrikson, R.C. Lanthanum binding by mouse cecal mucosa. Cell Tissue Res. 148, 309–311 (1974). https://doi.org/10.1007/BF00224259
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00224259