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Influence of castration on incorporation of exogenous peroxidase into “synaptic” vesicles of the median eminence

II. The perivascular part of the palisade zone in male rats

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Summary

In the median eminence of male rats, nerve profiles in the immediate vicinity of portal capillaries have been divided into 4 categories on the basis of their vesicular content: profiles a with agranular “synaptic” vesicles of about 50 nm, b with similar agranular vesicles and also with granular vesicles of mainly 60–140 nm, c with granular vesicles alone and d without vesicles.

Twenty-four hours after castration, the percentage of profiles of category a was significantly increased when compared with sham-operated animals, whereas the percentage of profiles of category b was significantly decreased. After intravenous injection of exogenous peroxidase, especially the nerve profiles located in direct contact with the outer basement membrane of the portal capillaries contained peroxidase positive “synaptic” vesicles. Injection off peroxidase after castration resulted in a significant increase in the percentage of nerve profiles containing both peroxidase positive and peroxidase negative “synaptic” vesicles (category a). It is suggested that, in certain nerve terminals, castration may lead to release off the content of granular vesicles, which may contain gonadotropin releasing factor. This release then may cause that nerve terminals with both agranular vesicles and granular vesicles (category b) change in their vesicular content and are therefore classified as terminals with only agranular vesicles (category a). An increased turnover rate in the ultrastructurally affected terminals may be reflected in increased uptake of exogenous peroxidase. The observations imply that certain neurones projecting to portal capillaries in the median eminence are, directly or indirectly, sensitive to changes in the level of gonadal steroids.

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Authors and Affiliations

  1. Department of Anatomy, Erasmus University, Rotterdam

    R. Stoeckart, H. G. Jansen & A. J. Kreike

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  1. R. Stoeckart
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  2. H. G. Jansen
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  3. A. J. Kreike
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The assistance of Mrs. R. M. Y. Hartsteen is gratefully acknowledged. We are also indebted to Miss P. C. Delfos and Mr. W. van den Oudenalder for photographic assistance, to Mrs. E. Jongbloed for assistance in preparing the manuscript and to Dr. J. H. Aafjes, Dr. D. A. Hopkins and Prof. J. Moll for helpful suggestions.

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Stoeckart, R., Jansen, H.G. & Kreike, A.J. Influence of castration on incorporation of exogenous peroxidase into “synaptic” vesicles of the median eminence. Cell Tissue Res. 159, 11–21 (1975). https://doi.org/10.1007/BF00231991

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