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Demonstration of the existence of a single morphological type of gonadotrophic cell in Ellobius lutescens (Microtinae) by an ultrastructural analysis of their development under various physiological and experimental conditions

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Summary

Ultrastructural studies of pituitaries from Ellobius lutescens (immature males and females, adult hypogonadic males, and virgin and pregnant females) show that the gonadotrophic cells are characterized by a lamellar or vacuolar rough endoplasmic reticulum (RER), a spirally-arranged Golgi apparatus, elongated mitochondria and secretory granules of variable density and size (150–500 mμ). Ultrastructural differences between gonadotrophic cells previously determined by light microscopy correspond to changes in the development of the protein synthetic apparatus and in the intensity of hormonal discharge. Type 2 gonadotrophs always appear to be more active than type 1 gonadotrophs. After castration, all gonadotrophic cells develop into the same form of “castration cell”, although type 1 gonadotrophs change more slowly than type 2 cells. Treatment with testosterone induces an inverse development of the gonadotrophic cells which take on the appearance of resting cells similar to those found in immature animals, where the two cell types are also identical. Thus, only one morphological type of gonadotrophic cell can be identified in Ellobius lutescens. Moreover, the gonadotrophic cells of the hypogonadic adult male have the same appearance as those of the female two months after castration, which proves that the negative feedback mechanism which regulates gonadotrophic function is defective in adult male Ellobius lutescens.

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References

  • Barnes, B.G.: Electron microscope studies on the secretory cytology of the mouse anterior pituitary. Endocrinology 71, 618–628 (1962)

    Google Scholar 

  • Barnes, B.G.: The fine structure of the mouse adenohypophysis in various physiological states. In: Cytologie de l'adénohypophyse, Colloque du C.N.R.S., 128, 91–110 (1963)

    Google Scholar 

  • Dekker, A.: Pituitary basophils of the Syrian hamster: an electron microscopic investigation. Anat. Rec. 158, 351–367 (1967)

    Google Scholar 

  • Della Corte, F., Angelini, F.: Dati ultrastrutturali su alcune modificazioni ipofisarie da castrazione, nel coniglio. Arch. zool. ital. 52, 221–235 (1967)

    Google Scholar 

  • Dubois, P., Girod, C.: Nouvelles recherches sur l'ultrastructure des cellules gonadotropes de l'hypophyse antérieure chez le Hamster doré. C.R. Soc. Biol. (Paris) 158, 2102–2104 (1965)

    Google Scholar 

  • Farquhar, M.G., Rinhart, J.F.: Electron microscope studies of the anterior pituitary gland of castrated rats. Endocrinology 54, 516–541 (1954)

    Google Scholar 

  • Farquhar, M.G., Rinhart, J.F.: Further evidence for the existence of two types of gonadotrophs in the anterior pituitary of the rat. Anat. Rec. 121, 394 (1955)

    Google Scholar 

  • Girod, C., Dubois, P.: Etude ultrastructurale des cellules gonadotropes antéhypophysaires chez le Hamster doré (Mesocricetus auratus). J. Ultrastruct. Res. 13, 212–232 (1965)

    Google Scholar 

  • Girod, C., Dubois, P., Curé, M.: Etude au microscope électronique des cellules gonadotropes antéhypophysaires du Hamster doré (Mesocricetus auratus) intact ou castré. C.R. Acad. Sci. (Paris) 258, 6536–6538 (1964)

    Google Scholar 

  • Herlant, M.: Apport de la microscopie électronique à l'étude du lobe antérieur de l'hypophyse, in: Cytologie de l'adénohypophyse. Colloque du C.N.R.S. 128, 73–90 (1963)

    Google Scholar 

  • Herlant, M.: Synthèse et excrétion des gonadostimulines. Localisation des activités gonadotropes au niveau du lobe antérieure de l'hypophyse. Arch. Anat. micr. Morph. exp. 56, suppl. 3–4, 424–445 (1967)

    Google Scholar 

  • Herlant, M., Ectors, F.: Les cellules gonadotropes de l'hypophyse chez le Porc. Z. Zellforsch. 101, 212–231 (1969)

    Google Scholar 

  • Kurosumi, K.: Functional classification of cell types of the anterior pituitary gland accomplished by electron microscopy. Arch. Histol. Jap. 29, 4, 329–362 (1968)

    Google Scholar 

  • Kurosumi, K., Oota, Y.: Electron microscopy of two types of gonadotrophs in the anterior pituitary glands of persistent estrous and diestrous rats. Z. Zellforsch. 85, 34–46 (1968)

    Google Scholar 

  • Lawzewitsch, I. von, Dickmann, G.H., Amezua, L., Pardal, C.: Cytological and ultrastructural characterization of the human pituitary. Acta anat. (Basel) 81, 286–316 (1972)

    Google Scholar 

  • Leleux, P., Robyn, C.: Etude en immunofluorescence d'une réaction immunologique croisée entre les gonadotropines humaines et les gonadotropines de Rat. Ann. Endocr. 31, 181–191 (1970)

    Google Scholar 

  • Mendoza, D., Arimura, A., Schally, A.V.: Ultrastructural and light microscopic observations of rat pituitary LH-containing gonadotrophs following injection of synthetic LH-RH. Endocrinology 92, 1153–1160 (1973)

    Google Scholar 

  • Nakane, P.K.: Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18, 9–20 (1970)

    Google Scholar 

  • Nakane, P.K.: Application of peroxidase-labelled antibodies to the intracellular localization of hormones. Acta endocr. (Kbh.) (suppl.) 153, 190 (1971)

    Google Scholar 

  • Nakayama, I., Nickerson, P.A., Skelton, F.R.: An electron microscopic study in ACTH- and FSH-producing cells during development of methylandrostenediol hypertension in the rat. Amer. J. Path. 58, 377–401 (1970)

    Google Scholar 

  • Phifer, R.F., Midgley, A.R., Spicer, S.S.: Immunohistologic and histologic evidence that follicle-stimulating hormone and luteinizing hormone are present in the same cell type in the human pars distalis. J. clin. Endocr. 36, 125–141 (1973)

    Google Scholar 

  • Rennels, E.G.: Electron microscopic alterations in the rat hypophysis after scalding. Amer. J. Anat. 17, 71–81 (1964)

    Google Scholar 

  • Rennels, E.G., Bogdanove, E.M., Arimura, A., Saito, M., Shcally, A.V.: Ultrastructural observations of rat pituitary gonadotrophs following injection of purified porcine LH-RH. Endocrinology 88, 1318–1326 (1971)

    Google Scholar 

  • Robyn, C., Leleux, P., Vanhaelst, L., Golstein, J., Herlant, M., Pasteels, J.L.: Immunohistochemical study of the human pituitary with anti-luteinizing hormone, anti-follicle stimulating hormone and anti-thyrotrophin sera. Acta endocr. (Kbh.) 72, 625–642 (1973)

    Google Scholar 

  • Salazar, H.: The pars distalis of the rabbit hypophysis: an electron microscopic study. Anat. Rec. 147, 469–497 (1963)

    Google Scholar 

  • Shiino, M., Rennels, E.G.: Ultrastructural observations on pituitary gonadotrophs following gonadectomy or administration of LH/FSH-releasing hormone in neonatal rats. Tex. Rep. Biol. Med. 32, 2, 561–567 (1974)

    Google Scholar 

  • Stefan, Y.: Les cellules à hormones glycoprotidiques de l'hypophyse d'un Rongeur d'Iran (Ellobius lutescens Th.): identification des cellules thyréotropes et gonadotropes et corrélations avec l'hypog énitalisme mâle. Gen. comp. Endocr. 19, 572–581 (1972)

    Google Scholar 

  • Stefan, Y., Dubois, M.P.: Mise en évidence par immunofluorescence des cellules à hormones glycoprotidiques dans l'hypophyse d'un Rongeur d'Iran, Ellobius lutescens (Th.): cellules gonadotropes et cellules thyréotropes. Z. Zellforsch. 129, 523–532 (1972)

    Google Scholar 

  • Stefan, Y., Dubois, M.P.: Comparative immunofluorescence studies of the gonadotropic cells in Ellobius lutescens using antibodies against LH and LHβ in normal, castrated and testosterone treated males. 8th Conf. of Eur. Comp. Endocrinologists, Bangor, Aug. 1975

  • Tougard, Cl., Kerdelhue, B., Tixier-Vidal, A., Justisz, M.: Localisation par cyto-immunoenzymologie de la LH, de ses sous-unités α et β et de la FSH dans l'adénohypophyse de la ratte. C.R. Acad. Sci. (Paris) 273, 897–900 (1971)

    Google Scholar 

  • Tougard, C., Kerdelhue, B., Tixier-Vidal, A., Justisz, M.: Light and electron microscope localization of binding sites of antibodies against ovine luteinizing hormone and its two subunits in rat adenohypophysis using peroxidase-labelled antibody technique. J. Cell Biol. 58, 3, 503–521 (1973)

    Google Scholar 

  • Tseng, M.T., Kittinger, G.W., Spies, H.G.: An ultrastructural study of the gonadotrophs in oophorectomized Rhesus (Macaca mulatta) adults treated with estrogen. Proc. Soc. exp. Biol. (N.Y.) 147, 412–417 (1974)

    Google Scholar 

  • Virgilis, G. de: Ultrastructure des cellules gonadotropes de l'adénohypophyse après ovariectomie. Ann. Endocr. 29, 553–561 (1968)

    Google Scholar 

  • Worth, R.W.: The electron microscopy of the adenohypophysis of the vole, Microtus agrestis. J. Physiol. 210, No. 1. (Proc. Physiol. Soc. 69–79) (1970)

  • Yamada, K., Nakamura, M., Yamashita, K.: Light and electron microscopic studies on the adenohypophysis of diabetic (KK) strain of the mouse. Z. Zellforsch. 79, 429–442 (1967)

    Google Scholar 

  • Yoshimura, F., Hammiya, K.: Electron microscopy of the anterior lobe of pituitary in normal and castrated rats. Endocr. jap. 12, 119–152 (1965)

    Google Scholar 

  • Yoshimura, F., Siji, T., Yachi, H., Ishikawa, H.: Life stage and secretory cycle of anterior pituitary basophils. Endocr. jap. 21, 217–250 (1974)

    Google Scholar 

  • Young, B.A., Foster, C.L., Cameron, E.: Ultrastructural changes in the adenohypophysis of castrated rabbits. J. Endocr. 35, 101–106 (1966)

    Google Scholar 

  • Zambrano, D., Deis, R.P.: The adenohypophysis of female rats after hypothalamic oestradiol implants: an electron microscopic study. J. Endocr. 47, 101–110 (1970)

    Google Scholar 

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  1. Y. Stefan

    Present address: Laboratoire d'Histologie et d'Embryologie, rue de l'Ecole-de-Médecine 20, 1205, Geneva, Switzerland

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  1. Laboratoire d'Endocrinologie, Geneva, Switzerland

    Y. Stefan

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Stefan, Y. Demonstration of the existence of a single morphological type of gonadotrophic cell in Ellobius lutescens (Microtinae) by an ultrastructural analysis of their development under various physiological and experimental conditions. Cell Tissue Res. 167, 49–64 (1976). https://doi.org/10.1007/BF00220159

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  • DOI: https://doi.org/10.1007/BF00220159

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