Summary
Using antibodies against mammalian LH-RH, the double antibody immunofluorescence technique has been applied to serial cross sections of the brains of adult Rana esculenta. Immunoreactive material was found in perikarya of an unpaired nucleus in front of the preoptic recess. The axons of these perikarya also contain fluorescing material. They form a single bundle which passes under the preoptic recess, than splits into two tracts, one on either side of the optic chiasm. The two tracts reunite just before entering the median eminence. The axons end near the capillaries in the outer zone of the median eminence. The possibility of two separate centres for the stimulation of gonadotropic activity in the brains of anurans is discussed.
Similar content being viewed by others
References
Barry, J., Dubois, M.P., Garette, B.: Immunofluorescence study of the preoptic infundibular LRF neuro-secretory pathway in the normal, castrated and testosterone treated male guinea pig. Endocrinology 95, 1416–1423 (1974)
Deery, D.J.: Determination by radioimmunoassay of the luteinising hormone-releasing hormone (LHRF) content of the hypothalamus of the rat and some lower vertebrates. Gen. comp. Endocr. 24, 280–285 (1974)
Dierickx, K.: Experimental identification of a hypothalamic gonadotropic centre. Z. Zellforsch. 74, 53–79 (1966)
Dierickx, K.: The function of the hypophysis without preoptic neurosecretory control. Z. Zellforsch. 78, 114–130 (1967)
Dierickx, K.: Identification of adenohypophysiotropic neurohormone producing cells in Rana temporaria. In: Neurosecretion — The final neuroendocrine pathway (Sir Francis Knowles and L. Vollrath, eds.), pp. 178–181. Berlin-Heidelberg-New York: Springer 1974
Dierickx, K., Vandesande, F.: The magnocellular preoptic nuclei and the reproduction in Rana temporaria. Z. Zellforsch. 68, 190–193 (1965)
Doerr-Schott, J., Dubois, M.P.: Immunoreactive LHRF neurons in the brain of Xenopus laevis. Gen. comp. Endocr. (in press) (1975a)
Doerr-Schott, J., Dubois, M.P.: Fixation de l'anticorps au niveau de la neurohypophyse du crapaud Bufo vulgaris. Laur. C.R. Acad. Sci. (Paris) 278, 1397–1399 (1975b)
Doerr-Schott, J., Dubois, M.P.: Localisation et identification d'un centre LH-RF dans l'encéphale du crapaud, Bufo vulgaris. Laur. C.R. Acad. Sci. (Paris) 280, 1285–1287 (1975c)
Goos, H.J.Th.: Hypothalamic neurosecretion and metamorphosis in Xenopus laevis. IV. The effect of extirpation of the presumed TRF cells and of a subsequent PTU treatment. Z. Zellforsch. 97, 449–458 (1969)
Goos, H.J.Th., Ligtenburg, P.I.M., van Oordt, P.G.W.J.: Immunohistochemical study on GTH-RH in the frog, Rana esculenta. Gen. comp. Endocr. (in press)
Holmes, R.L., Ball, J.N.: The pituitary gland. Series: Biological structure and function 4. Cambridge: Univ. Press 1974
Jeffcoate, S.L., Fraser, H.M., Gunn, A., Holland, D.T.: Radioimmunoassay of luteinizing hormone releasing factor. J. Endocr. 57, 189–190 (1973)
King, J.C., Parsons, J.A., Erlandsen, S.L., Williams, T.: Luteinizing hormone-releasing hormone (LH-RH) pathway of the ŕat hypothalamus revealed by the unlabeled antibody peroxydase antiperoxydase method. Cell Tiss. Res. 153, 211–218 (1974)
Licht, P., Papkoff, H.: Separation of two distinct gonadotrophins from the pituitary gland of the bullfrog Rana catesbiana. Endocrinology 94, 1587–1594 (1974)
Naik, D.V.: Immunoreactive LH-RH neurons in the hypothalamus identified by light and fluorescent microscopy. Cell Tiss. Res. 157, 423–436 (1975a)
Naik, D.V.: Immuno-electron microscopic localisation of luteinizing hormone-releasing hormone in the arcuate nuclei and median eminence of the rat. Cell Tiss. Res. 157, 437–455 (1975b)
Notenboom, C.D.: The relation between the caudo-dorsal region of the preoptic nucleus and the pars nervosa of the pituitary gland in Xenopus laevis tadpoles. Cell Tiss. Res. 149, 457–471 (1974)
Notenboom, C.D., Terlou, M.: Hypothalamic lesions and the ACTH-producing cells in the adenohypophysis of Xenopus laevis tadpoles. Gen. comp. Endocr. (in press)
Oordt, P.G.W.J.: Cytology of the adenohypophysis. In: Physiology of the amphibians, Vol. II (B. Lofts, ed.) pp. 53–106. London-New York: Acad. Press 1974
Oordt, P.G.W.J. van: The analysis and identification of the hormone-producing cells of the adeno hypophysis. In: Perspectives in endocrinology. Hormones in the lives of lower vertebrates (E.J.W. Barrington and C. Barker, Jørgensen, eds., pp. 405–467. London-New York: Acad. Press 1968
Oordt, P.G.W.J. van, Goos, H.J.Th., Peute, J., Terlou, M.: Hypothalamo-hypophysial relations in amphibian larvae. Gen. comp. Endocr., Suppl. 3, 41–50 (1972)
Oordt, P.G.W.J. van, Goos, H.J.Th., Peute, J., Terlou, M.: Structural and functional aspects of two types of Gomori-negative neurosecretory centres in the caudal hypothalamus of Amphibia. In: Neurosecretion The final neuroendocrine pathway (Sir Francis Knowles, L. Vollrath, eds.), p. 182–192). Berlin-Heidelberg-New York: Springer 1974
Peute, J., Mey, J.C.A.: Ultrastructural and functional aspects of the nucleus infundibularis ventralis in the green frog, Rana esculenta. Z. Zellforsch. 144, 191–217 (1973)
Peute, J., Oordt, P.G.W.J. van: The effect of testosterone treatment on the ultrastructure of the nucleus infundibularis ventralis of the green frog (Rana esculenta). In: Neurosecretion — The final neuroendocrine pathway (Sir Francis Knowles and L. Vollrath, eds.), p. 317. Berlin-Heidelberg-New York: Springer 1974
Schally, A., Arimura, A., Kastin, A.J.: Hypothalamic regulatory hormones. Science 179, 341–350 (1973)
Thornton, V.F., Geschwind, I.I.: Hypothalamic control of gonadotropin release in amphibia: evidence from studies of gonadotropin release in vitro and in vivo. Gen. comp. Endocr. 23, 294–301 (1974)
Vigh-Teichmann, I., Röhlich, P., Vigh, B.: Lichtund electronenmikroskopische Untersuchungen am Recessus praeopticus Organ von Amphibien. Z. Zellforsch. 98, 217–232 (1969)
Wheaton, J.E., Krulich, L., McCann, S.M.: Localisation of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus of the rat using radioimmunoassay. Endocrinology 97, 30–38 (1975)
Author information
Authors and Affiliations
Additional information
The authors wish to thank Mr. E. von der Vlist and Mr. J.J. van der Vlis for preparing the illustrations
Rights and permissions
About this article
Cite this article
Goos, H.J.T., Ligtenberg, P.J.M. & van Oordt, P.G.W.J. Immunofluorescence studies on gonadotropin releasing hormone (GRH) in the fore-brain and the neurohypophysis of the green frog, Rana esculenta L.. Cell Tissue Res. 168, 325–333 (1976). https://doi.org/10.1007/BF00215310
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00215310