Summary
Ovaries were removed from female rats and immediately autografted into a subcutaneous pouch in the flank in order to quantitate the relationship of graft re-innervation, steroid secretion and vaginal smear pattern. Animals were killed at three time periods: three days after grafting, on the first day a cornified vaginal smear appeared and at the first metestrus. In addition, control animals were killed at metestrus. Plasma samples were obtained from all rats and analyzed for estradiol-17β and progesterone concentration by radioimmunoassay.
At the first day of vaginal cornification after grafting, plasma estradiol-17β (45.8±4.0 pg/ml) was elevated in comparison to controls at metestrus (24.0±2.6 pg/ml), but plasma progesterone (21.5±4.0 ng/ml) was not different (30.6±1.7 ng/ml). Subsequently, at the first metestrus following grafting, plasma estradiol-17β (23.0±3.5 pg/ml) was comparable to control values. In contrast, progesterone was decreased (17.5±1.9 ng/ml). A definite correlation was detected between the vaginal smear and plasma levels of steroid hormones in the castrated female rat with subcutaneous ovarian autografts.
Histochemical techniques were used to study the adrenergic and cholinergic innervations of grafts three days after grafting, at the first day of vaginal cornification, and at the first metestrus. No correlation was shown between density of adrenergic or cholinergic innervation and plasma levels of estradiol-17β and progesterone or onset of a cycling vaginal smear.
Similar content being viewed by others
References
Burden, H.W.: Adrenergic innervation in ovaries of the rat and guinea pig. Amer. J. Anat. 133, 455–461 (1972)
Butcher, R.L., Collins, W.E., Fugo, N.W.: Plasma concentration of LH, FSH, prolactin, progesterone and estradiol-17β throughout the 4-day estrous cycle of the rat. Endocrinology 94, 1704–1708 (1974)
Chihal, H.J.W., Bast, J.D., Stone, S.C., Peppler, R.D.: Plasma estradiol-17β progesterone, FSH, LH and follicular development in castrated female rats with subcutaneous ovarian autografts. Biol. Reprod. 15, 173–178 (1976)
Deanesly, R.: Cyclic function in ovarian grafts. J. Endocr. 13, 211–220 (1956)
El-Badawi, A., Schenk, E.A.: Histochemical methods for separate, consecutive, and simultaneous demonstration of acetylcholinesterase and norepinephrine in cryostat sections. J. Histochem. Cytochem. 15, 580–588 (1967)
Falck, B., Owman, C.: A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. Lund. 2, 5–23 (1965)
Harris, M.A., Eakin, R.M.: Survival of transplanted ovaries in rats. J. exp. Zool. 112, 131–163 (1949)
Jacobowitz, D., Laties, A.M.: Adrenergic reinnervation of the cat ovary transplanted to the anterior chamber of the eye. Endocrinology 86, 921–924 (1970)
Jacobowitz, D., Wallach, E.E.: Histochemical studies of the autonomic innervation of the ovary. Endocrinology 81, 1132–1139 (1967)
Jordan, S.M.: Autonomic innervation of the ovary. J. Anat. (Lond.) 110, 500 (1971)
Kawashima, S., Mori, T.: Behavior of ovaries subcutaneously transplanted to ovariectomized hosts from normal or neonatally estrogenized donors. Proc. Japan Acad. 47, 76–80 (1971)
Pfeiffer, C.: Sexual differences in the hypophyses and their determination by the gonads. Amer. J. Anat. 58, 195–224 (1936)
Thorneycroft, I.H., Stone, S.C.: Radioimmunoassay of serum progesterone in women receiving oral contraceptive steroids. Contraception 5, 129–146 (1972)
Weiner, S., Wright, K.H., Wallach, E.E.: Selective ovarian sympathectomy in the rabbit. Fertil. Steril. 26, 353–362 (1975)
Author information
Authors and Affiliations
Additional information
Supported in part by USPHS Grant T01-DE00241-04
The authors wish to thank J. Canale, S. Hemelt, E. Schwartz and J. Skaggs for their technical and secretarial assistance. Anti-estradiol-17β antibody was obtained from Dr. I.H. Thorneycroft, University of Southern California School of Medicine, and anti-progesterone antibody from Dr. D. Tulchinsky, Harvard Medical Center
Rights and permissions
About this article
Cite this article
Chihal, H.J.W., Weitsen, H.A., Stone, S.C. et al. Autonomic innervation and plasma estradiol-17β and progesterone levels in rats with subcutaneous ovarian autografts. Cell Tissue Res. 175, 113–121 (1976). https://doi.org/10.1007/BF00220827
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220827